海藻糖快速鉴定光滑念珠菌方法评价  

Evaluation of the rapid trehalose test for the identification of the Candida glabrata

作　　者：何淳[1] 林琴[1] 包振英[1] 孟彦宏 齐小梅[1] 华红[2] 闫志敏[2] 

机构地区：[1]北京大学口腔医学院·口腔医院检验科,北京100081 [2]北京大学口腔医学院中医粘膜科,北京100081

出　　处：《北京大学学报（医学版）》2015年第1期186-190,共5页Journal of Peking University:Health Sciences

基　　金：北京大学口腔医院临床新技术新疗法项目(PKUSSNCT-15B15)资助~~

摘　　要：目的:以分子生物学鉴定结果为"金标准",通过两种不同方法对疑为光滑念珠菌(Candida glabrata)进行鉴定及检测结果比对,探讨一种便于临床采用、快速且廉价的检测光滑念珠菌的方法。方法:收集近3年真菌培养阳性菌株,选出在科玛嘉(CHROMagar)显色培养基孵育48 h后疑为光滑念珠菌200株,比较科玛嘉显色结果,经过37℃和42℃分别孵育3 h、6 h、24 h海藻糖发酵试验和分子生物学鉴定待测菌株到种。分析不同待测菌株对于发酵海藻糖的能力,评价海藻糖快速鉴定光滑念珠菌方法的最佳时间和温度,评估并比较科玛嘉显色和海藻糖发酵试验对于鉴定光滑念珠菌的诊断效能。结果:200株待测菌株中,仅有光滑念珠菌有快速发酵海藻糖的能力,42℃孵育3 h为海藻糖发酵试验的最佳条件。快速海藻糖发酵试验的正确率为99.00%(198/200),敏感性和特异性可以达到98.66%(147/149)和100.00%(51/51),相比较而言,科玛嘉显色对于鉴定光滑念珠菌的正确率为79.50%(159/200),敏感性和特异性仅为89.93%(134/149)和49.02%(25/51)。与目前国内经常使用的方法比较,海藻糖发酵试验具有更好的时间-效益比值。结论:快速海藻糖发酵试验操作简便,并且可以在3 h获得鉴定结果,费用低廉,具有在临床实验室推广开展的价值。Objective： To explore a rapid and cost-effective method for identification of Candida glabrata through the comparison of two different methods,using molecular methods of sequencing as gold standard. Methods： From our clinic,200 strains of suspected Candida glabrata were collected during the last 3 years and selected after incubation in CHROMagar Candida medium for 48 h. By comparing the results of the CHROMagar Candida medium,the identification of the rapid trehalose test for different kinds of strains were analyzed under incubation in the tubes for 3 h,6 h,and 24 h at 37 ℃ and 42 ℃,respectively. All the strains were identified to species level by methods of sequencing. The optimal time and temperature of the trehalose test for the identification of Candida glabrata were assessed. Two different methods,CHROMagar Candida medium and the rapid trehalose test,in identification of Candida glabrata were compared. Results： In all the 200 strains,Candida glabrata ferment trehalose with 3 h incubation under 42 ℃ were the optimal time and temperature for fermenting trehalose. The accuracy,sensitivity,and specificity of the rapid trehalose test were 99. 00%（ 198 /200）,98. 66%（ 147 /149）and 100. 00%（ 51 /51）. The accuracy rate of CHROMagar Candida medium was 79. 50%（ 159 /200）,the sensitivity and specificity were only 89. 93%（ 134 /149） and 49. 02%（ 25 /51）,however,compared with the domestic current popular methods,the rapid trehalose test had better time efficiency ratio. Conclusion： The evaluation results suggest that the rapid trehalose test has advantages in terms of operational convenience and low cost,and the results can be obtained in 3 h. Therefore,it has application value in clinical laboratory.

关 键 词：念珠菌 光滑 海藻糖 实验室技术和方法 

分 类 号：R379.4[医药卫生—病原生物学]