实时荧光PCR法特异性检测棉花曲叶病毒  

Rapid detection of cotton leaf curl virus by real-time PCR

作　　者：张卫东[1] 王岚[1] 冯宇梅[1] 廖力[1] 权永兵[1] 水克娟[1] 徐淼锋[1] 迟远丽[1] 黄永辉[1] 

机构地区：[1]珠海出入境检验检疫局技术中心,广东珠海519015

出　　处：《广东农业科学》2015年第2期126-130,136,共6页Guangdong Agricultural Sciences

基　　金：国家质量监督检验检疫总局课题(2012IK300);珠海出入境检验检疫局项目(ZH2012-4)

摘　　要：根据棉花曲叶病毒(Cotton leaf curl virus,CLCuV)运动蛋白基因序列特征,设计并合成了特异性Taqman探针和检测引物,建立该病毒的实时荧光PCR快速检测方法。结果表明:该方法可以特异性地从5个不同CLCuV分离物中扩增到荧光信号,而其他6种病毒均无扩增。建立的方法具有快速、准确、灵敏及简便等特点,可为进出境植物检疫和农业安全生产提供可靠的技术支持。In this study,special Taqman fluorescent probes and primers weredesigned based on the sequence of movement protein gene,a rapid and effective method of cotton leaf curl virus（CLCuV）detection was established based on real-time fluorescent PCR.The result showed that the method could amplify specifically fluorescence signal from 5different isolates of CLCuV,while the other 6 viruses had no amplification.The method is not only rapid,accurate,convenient,and sensitive,but also can provide well technical support for Entry-Exit Inspection and Quarantine and agricultural safety.

关 键 词：棉花曲叶病毒(CLCuV) 运动蛋白基因 实时荧光PCR技术 

分 类 号：S432.41[农业科学—植物病理学]