不同外源激素及活性炭的添加对白芨增殖的影响  

The Effect of the Addition of Different Exogenous Hormones and Activated Carbon on Bletilla striata Proliferation

作　　者：江胜德 郭兰 王邝佳 杨兰兰 

机构地区：[1]江苏省昆山虹越花卉有限公司,江苏昆山215300 [2]浙江省杭州虹越花卉有限公司,浙江杭州310000

出　　处：《安徽农业科学》2015年第9期24-25,52,共3页Journal of Anhui Agricultural Sciences

基　　金：江苏省农业科技支撑计划项目"白芨与野生黄花白芨远缘杂交新品种选育及组培苗工厂化生产与示范项目(BE2014323);江苏省昆山市农业科技计划项目"药用观赏花卉白芨组培苗无菌检测技术研究及产业化开发"(KN1319);浙江省农业科技成果转化项目"基于无菌检测的药用花卉白芨组培苗的工厂化生产与示范"

摘　　要：[目的]研究不同外源激素配比以及不同量的活性炭添加对白芨丛生芽增殖的影响,以实现不同外源激素对白芨增殖的配比优化。[方法]以MS为基本培养基,通过细胞分裂素(6-BA)和生长素(NAA)的不同配比添加,研究其对白芨组培苗增殖的影响。[结果]当6-BA 2.0 mg/L+NAA 0.2 mg/L时,白芨增殖系数最高,达4.00;另外,活性炭添加可防止白芨苗褐化,但当添加量为0.20 g/L时,不仅有效地抑制了白芨组培苗的褐化,而且对白芨的生长分化影响最小。[结论]适合白芨增殖的培养基为MS+6-BA 2.0 mg/L+NAA0.2 mg/L,活性炭添加量为0.20 g/L。[Objective] The aim was to study the effect of the addition of different exogenous hormones and activated carbon on Bletilla striata proliferation,in order to achieve proportioning optimization of different exogenous hormones on Bletilla striata proliferation. [Method] With MS as the basic culture medium,by adding the different proportion of cytokinin（ 6-BA） and auxin（ NAA）,the effect of the addition of different exogenous hormones and activated carbon on Bletilla striata proliferation was studied. [Result]6-BA and NAA in accordance with the ＂6-BA 2. 0 mg / L ＋ NAA 0. 2 mg / L ratio,coefficient of Bletilla striata proliferation was highest with 4. 00; In addition,adding activated carbon could prevent Bletilla striata browning,but when the content was 0. 20 g / L,it not only effectively suppress Bletilla striata browning,but also did not affect the growth of Bletilla striata. [Conclusion] Bletilla striata proliferation medium was MS ＋ 6-BA 2. 0 mg / L ＋ 0. 2 mg / L NAA,and activated carbon content was 0. 20 g / L.

关 键 词：白芨 组织培养 丛生芽增殖 活性炭 

分 类 号：S567.1[农业科学—中草药栽培]