Cu^(2+)对铜锈微囊藻生长及叶绿素荧光特性的影响  

Effects of Cu^(2+) on the Growth and Chlorophyll Fluorescence of Microcystis aeruginosa

作　　者：晋利[1] 李晓亮[1] 

机构地区：[1]中国环境管理干部学院环境工程系,河北秦皇岛066102

出　　处：《植物生理学报》2015年第2期178-182,共5页Plant Physiology Journal

基　　金：国家自然科学基金(41201244);中国环境管理干部学院博士基金(B201409)

摘　　要：利用浮游植物荧光仪(Photo-PAM)研究了不同Cu^(2+)浓度处理不同时间(12、24、48、72、96 h)对铜锈微囊藻(Microcystis aeruginosa)生长及叶绿素荧光特性的影响。结果表明:在一定时间(96 h)内,低浓度Cu^(2+)(1μmol·L^(-1))处理的铜锈微囊藻的藻细胞密度以及PSII最大光合效率(Fv/Fm)、PSII实际光合效率(ΦPSII)、光能利用效率(α)与对照相比变化不显著,最大电子传递速率(Pm)显著下降;高浓度Cu^(2+)(2、4、6、8μmol·L^(-1))处理下,藻细胞密度及Fv/Fm、ΦPSII、α、Pm随着Cu^(2+)浓度的加大和胁迫时间的延长均显著降低,浓度为6、8μmol·L^(-1) Cu^(2+)处理48 h,铜锈微囊藻Fv/Fm、ΦPSII、α、Pm的测得值为0。可见,高浓度Cu^(2+)(≥2μmol·L^(-1))处理极大地影响铜锈微囊藻的生长及叶绿素荧光特性,胁迫作用显著。Algae, live in body of water, are inevitably stressed by copper pollution. The growth and chlorophyll fluorescence traits of Microcystis aeruginosa exposed to the culture medium with different copper concentra- tions for different times （12, 24, 48, 72, 96 h） were studied. The results showed that the maximal photochemical effi- ciency of PSII （Fv/Fm）, the actual photochemical efficiency of PSII in the light （φPSII） and the light use efficiency （α） were no significant differences under low CuE＋ concentration （1 μmol· L-1）, but the maximal relative electron transport rate （Pro） was decreased significantly within 96 hours. However, when CuE＋ concentrations were high （2, 4, 6 and 8 μtmol·L-l）, the differences of the parameters were significant. And the changes of the decrease tendency were evident with the increase of Cu2＋ concentrations and the prolongation of time. The measured values of Fv/Fm, φPSII, α and Pm were zero when Cu2＋ concentrations were 6 and 8 μmol·L-1 after 48 h. Therefore, the high content of Cu2＋ （≥2 μmol·L-1） inhibited the growth and chlorophyll fluorescence of M. aeruginosa severely.

关 键 词：CU2+ 铜锈微囊藻 生长 叶绿素荧光 

分 类 号：Q945[生物学—植物学]