诸氏鲻虾虎鱼多态性微卫星标记的开发及评价  

Isolation and characterization of polymorphic microsatellite markers in Mugilogobius chulae

作　　者：蔡磊[1] 陈小曲[1] 郑伟强[1] 李建军[1] 

机构地区：[1]广东省实验动物监测所、广东省实验动物重点实验室,广州510663

出　　处：《中国实验动物学报》2015年第1期57-62,共6页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家科技支撑计划项目(2013BAK11B02);广东省中国科学院全面战略合作项目(2011B090300099);广东省科技计划项目(2012A020602008);广东省科技计划项目(2011B010500021)

摘　　要：目的开发诸氏鲻虾虎鱼(Mugilogobius chulae)多态性微卫星标记,为诸氏鲻虾虎鱼的遗传背景评价提供基础数据。方法采用磁珠富集法,以生物素标记的(AC)15为探针,构建了诸氏鲻虾虎鱼微卫星富集文库。经克隆、筛选、测序后,设计合成微卫星引物,利用野生群体对微卫星引物进行多态性筛选和评价。结果共获得74个含有微卫星重复单元的序列,根据微卫星序列共设计出33对微卫星引物。利用30个野生诸氏鲻虾虎鱼样本对33对引物的微卫星位点进行了评价,其中有12个位点表现出多态性,平均有效等位基因数(Ne)、观测杂合度(Ho)、期望杂合度(He)及多态信息含量(PIC)分别为2.9167、2.2311、0.4583、0.5633和0.4474。结论所筛选的微卫星标记将为诸氏鲻虾虎鱼遗传质量控制及监测提供实验依据。Objective To isolate microsatellite DNA markers from genome of Mugilogobius chulae for further identi- fication of its genetic background. Methods A microsatellite-enriched genomic library for Mugilogobius chulae was con- structed by magnetic-bead enrichment method using biotinylated （AC）15probe. After a series of steps, the obtained microsat- ellite markers were assessed and evaluated versus that of wild population of Mugilogobius chulae. Results 74 repeated se- quences containing microsatellite sequences were obtained from 95 randomly picked positive clones. 33 primers were designed and synthesized. All of these loci were selected for polymorphism analysis in a wild population of Mugilogobius chulae, and 12 of them showed polymorphism. The mean number of alleles （Na）, the number of effective alleles （Ne） , observed heterozy- gosity （Ho）, expected heterozygosity （ He）, and polymorphic information content （PIC） were 2. 9167, 2. 2311, 0. 4583, 0. 5633 and 0. 4474, respectively. Conclusions The microsatellites developed in this study represent the first nuclear mark- ers described in Mugilogobius chulae and will be useful tools for genetic studies of Mugilogobius chulae.

关 键 词：诸氏鲻虾虎鱼 多态性 微卫星标记 遗传多样性 

分 类 号：Q95-33[生物学—动物学]