小鼠DETCs表型及功能的初步研究  

Preliminary study for the phenotypes and functions of murine DETCs

作　　者：燕荣帅[1] 黄华[1] 刘美希[1] 胡晓红[1] 张小容[1] 罗高兴[1] 贺伟峰[1] 吴军[1] 

机构地区：[1]第三军医大学西南医院全军烧伤研究所创伤、烧伤与复合伤国家重点实验室重庆市疾病蛋白质组学重点实验室,重庆400038

出　　处：《免疫学杂志》2015年第3期214-217,共4页Immunological Journal

基　　金：国家自然科学基金(81372082,81373155)

摘　　要：目的通过分析小鼠表皮内T淋巴细胞(dendritic epidermal T cells,DETCs)的形态、表型及细胞因子的表达情况,揭示小鼠DETCs活化后能够发挥抗原递呈功能。方法通过免疫荧光的方法观察DETCs的形态,通过流式细胞技术分析DETCs的表型及细胞因子的表达情况。通过DETCs和na觙ve CD4+T细胞进行共培养,观察DETCs对淋巴细胞分化方向的影响。结果 DETCs在形态上表现出抗原递呈细胞的树突状特征,活化后分泌一定水平的IFN-γ,DETCs活化后在体外与na觙ve CD4+T细胞共培养可以诱导该型T细胞向Th1方向分化。结论小鼠DETCs具有抗原递呈细胞的基本特征,是皮肤免疫微环境的重要组成部分。To preliminarily clarify wether the murine DETCs are able to function as antigen-presenting cells,the general morphology of DETCs was observed by immunofluorescence, while the phenotypes and the secreted cytokines of DETCs were analyzed by flow cytometry. Furthermore, the differentiation of naive CD4＋T cells, which co-cultured with the DETCs in vitro, was identified by flow cytometry. DETCs showed morphologically a dendritic shape of antigen-presenting cells, and could secrete a certain level of IFN-γ uponstimulation ex vivo. Moreover,DETCs were determined to be able to induce Th1 differentiation of naive CD4＋T cells in vitro. In conclusion, the murine DETCs, as an important part of the skin immune microenvironment, can function as antigen-presenting cells.

关 键 词：ΓΔT细胞 DETC IFN-Γ 抗原递呈 

分 类 号：R392.11[医药卫生—免疫学]