IL-31对小鼠肺泡上皮细胞趋化因子表达的影响  

IL-31 induces the expression of chemokines in mouse alveolar epithelial cells

作　　者：岳欢[1,2] 高婧[1] 江涛[1] 高雅倩[1] 黄俊琼[1] 

机构地区：[1]遵义医学院附属医院检验科,563003 [2]遵义市第一人民医院检验科,563000

出　　处：《免疫学杂志》2015年第3期218-220,229,共4页Immunological Journal

基　　金：国家自然科学基金(81260266);贵州省优秀科技教育人才省长专项基金(黔省专合字200951)

摘　　要：目的体外分离培养小鼠Ⅱ型肺泡上皮细胞,探讨IL-31对肺泡上皮细胞表达趋化因子的影响。方法酶消化法分离及免疫吸附法纯化小鼠Ⅱ型肺泡上皮细胞,透射电子显微镜观察细胞形态及结构,免疫荧光技术检测细胞SP-A的表达。用100 ng/ml IL-31作用小鼠Ⅱ型肺泡上皮细胞24 h后提取细胞RNA,PCR芯片检测小鼠肺泡上皮细胞趋化因子及其受体的表达。结果透射电镜观察发现细胞内存在板层小体,呈同心圆状或平行排列;激光共聚焦显微镜观察可见细胞内有黄绿色荧光;小鼠肺泡上皮细胞经100 ng/ml IL-31作用24 h后,基因芯片检测细胞中表达上调的趋化因子和细胞因子基因11个、受体基因8个。结论成功分离培养小鼠Ⅱ型肺泡上皮细胞,IL-31可诱导肺泡上皮细胞表达趋化因子,表明IL-31可通过诱导趋化因子参与气道炎症反应。To investigate the effect of IL-31 on the expression of chemokines in alveolar epithelial cells, type II alveolar epithelial cells were treated with 100 ng/ml of IL-31, then total RNA was extracted and chemokines as well as their receptors m RNA were analyzed by PCR microarray. We found that 11 chemokines and 8 receptors were up-regulated, while 8 chemokines and 5 receptors were down-regulated in alveolar epithelial cells after treatment with interleukin 31, compared with those in control group. The results suggested that IL-31 can induce the expression of chemokines in alveolar epithelial cells in mice, indicating that IL-31 may play an important role in airway inflammation of asthma through induction of chemokines expression in lung tissue.

关 键 词：肺泡上皮细胞 白细胞介素31 趋化因子 基因芯片 

分 类 号：R562.25[医药卫生—呼吸系统]