MALDI Biotyper和VITEKMS基质辅助激光解析电离飞行时间质谱对链球菌鉴定的比较研究  

Evaluation of MALDI Biotyper and VITEK MS for identifying clinical isolated Streptococcus spp.

作　　者：顾丹霞[1] 余涛[1] 张晓飞[1] 张嵘[1] 

机构地区：[1]浙江大学医学院附属第二医院检验科,杭州310009

出　　处：《中华检验医学杂志》2015年第2期98-101,共4页Chinese Journal of Laboratory Medicine

摘　　要：目的以16SrRNA基因测序鉴定结果为金标准，研究比较两种基质辅助激光解析电离飞行时间质谱鉴定系统（MALDI Biotyper和VITEKMS）在临床分离链球菌鉴定中的表现。方法2014年4至6月收集浙江大学医学院附属第二医院临床分离的链球菌162株，经16SrRNA基因测序确认菌种，分别使用MALDI Biotyper和VITEKMS质谱鉴定系统进行鉴定，并评价其鉴定能力。结果MALDI Biotyper鉴定系统将155株（155／162，95．68％）链球菌准确鉴定到种，将3株缓症链球菌群鉴定为肺炎链球菌，1株副血液链球菌鉴定为澳大利亚链球菌，另有3株肺炎链球菌不能准确鉴定（分值〈1．7）；VITEKMS鉴定系统将156株（156／162，96．30％）链球菌准确鉴定至种（包括亚种），其将2株肺炎链球菌鉴定为缓症链球菌／口腔链球菌，1株马肠链球菌鉴定为婴儿链球菌婴儿亚种。两系统对化脓性链球菌和无乳链球菌的鉴定准确率均为100％（51／51），对肺炎链球菌的准确率均〉85％。结论两系统对临床分离的链球菌均有较强的鉴定能力，VITEKMS系统对某些亚种的准确鉴定更有临床意义。质谱可作为临床链球菌快速鉴定的手段。Objective Using 16S rRNA gene sequencing as the gold standard method, to compare the performance of two matrix-assisted laser desorption ionization time of flight mass spectrometry system （MALDI Biotyper and VITEK MS） for identifying clinical isolates of Streptococcus spp. Methods One hundred and sixty two clinical Streptococcus isolates were collected at the Second Affiliated Hospital of Zhejiang University, from April to June, 2014, and confirmed by 16S rRNA gene sequencing analysis. MALDI Biotyper and VITEK MS mass spectrometry system were used for identification and further evaluated by performance respectively. Results Of all the isolates tested, 155 （ 155/162, 95.68% ） Streptococcus isolates were accurately identified to species level by MALDI Biotyper. Besides, MALDI Biotyper identified three Streptococcus mitis group as S. pneunmniae and one S. parasanguinis as S. australis. Another three S. pneumonia isolates were not identified accurately （ values 〈 1.7 ）. Although 156 （ 156/ 162, 96. 30% ） isolates were accurately identified to species level （including subspecies） by VITEK MS system, two S. pneumoniae as S. mitis/S, oralis and one S, euinus as S. infantarius ssp. infantarius were misidentified. The two systems showed a 100% （51/51） accuracy in identifying all S. pyogenes and S. agalactiae isolates, and an accuracy higher than 85% for S. pneumoniae. Conclusions Both systems showed potent identification ability for Streptococcus spp., VITEK MS system showed more clinical significance in accurately identifying some subspecies. Mass spectrometry system can be used as a rapid identification method for Streptococcs spp. in clinical practice.

关 键 词：链球菌科 RNA 核糖体 16S 细菌分型技术 

分 类 号：R446.5[医药卫生—诊断学]