NLRP3炎性小体及下游因子IL-1β／IL-18在变应性鼻炎大鼠模型中的表达及意义  

Expression and significance of NLRP3 inflammasome and its downstream factors IL-1β/IL-18 in rat model of allergic rhinitis

作　　者：万慧娟[1] 苏红霞[1] 吴玉瑛[1] 赵玉林[1] 周明辉[1] 

机构地区：[1]郑州大学第一附属医院鼻科河南省高等学校临床医学重点学科开放实验室,450052

出　　处：《中华耳鼻咽喉头颈外科杂志》2015年第2期145-150,共6页Chinese Journal of Otorhinolaryngology Head and Neck Surgery

基　　金：河南省自然科学基金（112102310160）

摘　　要：目的检测天然免疫因子NLRP3炎性小体及下游因子白细胞介素1β（IL-1β）／IL-18在变应性鼻炎（AR）大鼠模型中的表达情况及其意义。方法40只健康sD大鼠，雌雄不限，随机数字表法分为对照组（A组）、AR模型1组（B组）、AR模型2组（C组）、AR模型3组（D组），每组10只。A组不加卵清蛋白，B、C、D组用卵清蛋白及氢氧化铝基础致敏后，用5％卵清蛋白生理盐水溶液分别滴鼻10、20、30d，1次／d，激发完成记录大鼠鼻部症状并处死大鼠。苏木精-伊红（HE）染色观察嗜酸粒细胞（EOS）计数、免疫组化观察NLRP3、半胱氨酸天冬酶1（Caspase-1）在各组大鼠鼻黏膜的表达情况，酶联免疫吸附实验（ELISA）检测大鼠卵清蛋白特异性IgE（OVA—slgE）、IL-18、IL-1β在各组大鼠外周血中的相对表达量及IL-1β、IL-18在各组大鼠鼻腔冲洗液中的含量。以SPSS17．0软件进行统计学分析。结果嗜酸粒细胞（EOS）计数、行为学评分、OVA—sIgE浓度在AR模型组高于对照组，差异有统计学意义（P值均〈0．05），AR模型组之间比较差异亦有统计学意义（P值均〈0．05）。NLRP3在B、C、D组鼻黏膜中的相对表达量分别为48．80±10．75、71．80±16．98、100．32±13．91，高于对照组的17．47±5．59，差异有统计学意义（F=78．399，P〈0．05）；Caspase-1在B、c、D组鼻黏膜中的相对表达量分别为36．33±4．71、50．87±11．18、73．10±14．77，高于对照组的11．48±2．70，差异有统计学意义（F=71．727，P〈0．05）；IL-1β在B、C、D组外周血中的含量分别为（56．46±10．13）、（82．37±11．93）、（112．01±22．91）pg／ml，高于对照组的（38．26±4．66）pg／ml，差异有统计学意义（F=51．981，P〈0．05）；IL-18在B、C、D组外周血中的含量分别为（177．92±23．63）、（194．33±20．78）、（234．06±31．70）pg／ml，高于对照组的（89．71±5�Objective To detect the expression and explore the role of the innate immune NLRP3 inflammasome and its downstream factors interleukin-1β （IL-1β）/ interleukin-18 （IL-18） in rat model of allergic rhinitis（AR）. Methods Forty Sprague Dawley （SD） rats were randomly divided into control group （ A group） , AR model group 1 （ B group） , AR model group 2 （ C group） , AR model group 3 （ D group）. Every group contained 10 rats. After the rats in the model group were sensitized by ovalbumin （OVA） and alum, B, C and D groups were separately stimulated with 5% OVA for 10 days,20 days and 30 days （once a day）. The control group did not add OVA in the process of sensitization and excitation. All rats were executed after excitation. Eosinophil granulocyte （EOS） infiltration were observed in nasal mucosa by hematoxylin-eosin （HE） staining, the expression of NLRP3 and cysteinyl aspartate-specific protease-1 （Caspase-1） were observed in nasal mucosa by immunohistochemical staining. The concentrations of ovalbumin specific IgE （OVA-sIgE） , IL-18 and IL-1β in peripheral blood and the concentrations of IL-18 and IL-1β in nasal fluid were tested by enzyme-linked immunosorbent assay （ELISA）. The data were processed by SPSS 17.0 software. Results EOS cell counted, the behavioral score and the concentrations of OVA-sIgE in AR model group were obviously higher than those in control group （P 〈 0. 05 ） , and the difference of which had statistical significance between the AR model groups （ P 〈 0. 05 ）. The expression of NLRP3 in AR model group （The expression of NLRP3 in group of B,C and D were 48.80 ± 10. 75,71.80 ± 16. 98 and 100. 32± 13.91 ,respectively）were obviously higher than those in control group （17.47 ± 5.59 ）, the difference of which had statistical significance （F = 78. 399 ,P 〈 0. 05 ）. The expression of Caspase-1 in AR model group （The expression of Caspase-1 in group of B,C and D were 36.33 ±4.71,50.87 ± 11.18 and 73. 10 ± 14. 7

关 键 词：鼻炎 变应性 受体 胞质和核 半胱氨酸天冬氨酸蛋白酶1 白细胞介素1Β 白细胞介素18 疾病模型 动物 

分 类 号：R765.21[医药卫生—耳鼻咽喉科]