左归丸对衰老大鼠骨髓MSC形态学影响的研究  

Effect of Zuogui Pill on aging rat MSC Morphology

作　　者：詹菲[1] 张晨[1] 黄进[1] 徐志伟[1] 罗川晋[1] 丁富平[1] 李杰斌[1] 张进[1] 

机构地区：[1]广州中医药大学,广东广州510405

出　　处：《时珍国医国药》2015年第3期513-515,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.81202732;No.30500645);广东省普通高校重点实验室开放基金资助项目(No.AAF433111A27)

摘　　要：目的研究补肾益精代表方左归丸对衰老大鼠骨髓间充质干细胞(MSC)形态学方面的影响。方法通过腹腔注射D-半乳糖造模,制备衰老大鼠模型,分离培养骨髓MSC。光镜观察各组大鼠MSC一般形态学特征,电镜观察其超微结构特点。结果光镜观察模型组P3代大鼠骨髓MSCs呈现衰老细胞形态学特征,左归丸组大鼠BMSCs的细胞形态与正常组相近。透射电镜观察显示,模型组大鼠P3代骨髓MSCs细胞内结构改变呈现衰老特征,左归丸中剂量组大鼠的骨髓MSCs电镜下的超微结构与正常组最相近。结论衰老大鼠骨髓MSCs的细胞形态各方面表现有明显改变,左归丸水提液在一定浓度范围内能延缓大鼠骨髓MSCs的衰老。Objective To investigate the effect of Zuogui pill on aging rats bone marrow mesenchymal stem cell（ MSC） morphology. Methods Aging rat model was made by intraperitoneal injection of D- galactose. MSCs were stained by Giemsa method,and were observed by the optical microscope and electron microscope respectively. Results Under the optical microscope,MSC of model group presented morphological characteristics of senescent cells,while those in Zuogui pill groups were close to the normal group. Under the electron microscope,MSC of model group presented senescent cells morphological characteristics,but the ultrastructure of the Zuogui pill groups BMSCs were close to the normal group. Conclusion The morphology of aging rat BMSC have obvious change,Zuogui pill which in a certain concentration range can delay the aging of rat BMSC.

关 键 词：左归丸 衰老 骨髓间充质干细胞 形态学 

分 类 号：R285.5[医药卫生—中药学]