四藤方对人胃癌SGC-7901细胞增殖及凋亡的影响  

The effect of Si-Teng-Fang on proliferation and apoptosis in gastric carcinoma SGC-7901 cells

作　　者：何萍[1] 沈克平[1] 胡兵[1] 梁云麒 

机构地区：[1]上海中医药大学附属龙华医院,上海200032

出　　处：《时珍国医国药》2015年第3期531-533,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.30873258);上海市卫生局中医药科研重点项目(No.2012J006A);上海市中医药领军人才项目;上海市级医院临床科研资源共享平台(No.SHDC12007206)

摘　　要：目的观察四藤方中中药及联合应用对人胃癌SGC-7901细胞增殖、凋亡的影响。方法体外培养胃癌SGC-7901细胞,不同浓度中药作用后,CCK-8法检测细胞增殖,流式细胞仪检测细胞凋亡;Caspase-3活性检测试剂盒检测SGC-7901细胞Caspase-3的活性。结果与对照组比较,四藤方中菝葜、红藤、藤梨根均能抑制SGC-7901细胞增殖。菝葜、红藤、藤梨根联用可增强对SGC-7901细胞增殖和凋亡的作用,同时提高SGC-7901细胞Caspase-3活性(P<0.01)。结论菝葜、红藤、藤梨根三药联合使用可增强对人胃癌SGC-7901的抗癌作用,并可能与活化Caspase-3介导细胞凋亡相关。Objective To observe the combinational effects of herbs in Si- Teng- Fang on proliferation and apoptosis in gastric carcinoma SGC- 7901 cells. Methods Human gastric carcinoma SGC- 7901 cells were cultured,and treated with different concentration herbs or herb combinations from Si- Teng- Fang. Cell proliferation was detected by Cell Counting Kit- 8 assay. Cell apoptosis was identified by flow cytometry analysis. The activities of caspase- 3 were detected by Caspase- 3 Activity Assay Kit.Results Compared with the control,Ba- qia,Hong- teng and Teng- li- gen significantly inhibited the proliferation of SGC-7901 cells. The combination of Ba- qia,Hong- teng and Teng- li- gen was found to be more effective than single herb alone in inhibiting proliferation and inducing apoptosis in SGC- 7901 cells（ P 0. 01）. In addition,caspase- 3 were significantly activated by combinational treatment of Ba- qia,Hong- teng and Teng- li- gen,and compared with either agent alone. Conclusion-Combination of Ba- qia,Hong- teng and Teng- li- gen may elicit enhanced anti- cancer effects in human gastric carcinoma SGC- 7901 cells,and may associated with the activation of caspase- 3 mediated apoptosis.

关 键 词：胃癌 中医药 四藤方 细胞凋亡 Caspases-3 

分 类 号：R285.5[医药卫生—中药学]