慢病毒介导miR-4258-siRNA表达对波动高糖诱导肾小管上皮细胞转分化的影响  

The Effect of miR-4258-siRNA-Lentivirus on Epithelial-Messenchymal Transition in HK-2 Induced by Intermittent High Glucose

作　　者：曹罗元[1] 林应华[1] 黄宝英[1] 富显果[1] 杨菁[1] 林峰[1] 

机构地区：[1]福建医科大学附属宁德市医院,宁德352100

出　　处：《中国细胞生物学学报》2015年第2期185-190,共6页Chinese Journal of Cell Biology

基　　金：福建医科大学科技发展专项基金(批准号:FZS13022Y);福建省卫计委青年科研课题(批准号:2013-1-50);宁德市科技计划项目(批准号:20130203;20130199)资助的课题~~

摘　　要：该实验构建了慢病毒介导(lentivirus,LV)的miR-4258-siRNA,探讨干扰miR-4258对肾小管上皮细胞(HK-2)转分化的影响。将HK-2转分化细胞分为正常组、阴性对照组和干扰组;荧光定量PCR方法检测miR-4258的表达情况;免疫印迹检测Fibronectin、E-cadherin和α-SMA的表达情况。结果显示,miR-4258-siRNA-LV可显著降低miR-4258的表达水平,干扰组的Fibronectin和α-SMA的相对表达量较阴性对照组显著下调;而E-cadherin的相对表达量较阴性对照组明显上调。研究说明,通过慢病毒介导的miR-4258-siRNA-LV下调miR-4258的表达,可抑制波动高糖诱导的HK-2细胞转分化过程。We constructd lentivirus-mediated miR-4258-siRNA and observed the effect of miR-4258-siRNALV on epithelial-messenchymal transition in HK-2 induced by intermittent high glucose. The HK-2 cells were divided into normal group, negative control and interference group. The expression of miR-4258 in HK-2 cells was detected by Real-time q PCR. The expressions of Fibronectin, E-cadherin and α-SMA were tested by Western blot. The results showed that the expression of mi R-4258 was decreased significantly by lentivirus-mediated mi R-4258-si RNA. The expression of Fibronectin and α-SMA were down-regulated markedly in interference group than those of negative control. Meanwhile, the expression of E-cadherin was up-regulated significantly in interference group than that of negative control. This study shows that mi R-4258-si RNA can suppress epithelial-messenchymal transition in HK-2 cells induced by intermittent high glucose.

关 键 词：miR-4258 上皮细胞转分化 HK-2 波动高糖 

分 类 号：R692.6[医药卫生—泌尿科学]