撕裂半月板中软骨退变相关基因及miRNAs的表达  

EXPRESSIONS OF CARTIL AGE DEGENER ATIVE REL ATED GENES AND micro RNAs IN TORN MENISCUS

作　　者：龙毅[1] 何爱珊[1] 张志奇[1] 孟繁钢[1] 侯昌禾[1] 张紫机[1] 黄广鑫[1] 廖威明[1] 

机构地区：[1]中山大学附属第一医院关节外科,广州510080

出　　处：《中国修复重建外科杂志》2015年第3期301-306,共6页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：国家杰出青年科学基金项目(81201388);广东省科技厅项目计划(2008B030301042)~~

摘　　要：目的研究半月板中软骨退变相关基因的表达,探讨半月板撕裂对软骨退变的潜在影响,并分析mi RNAs和软骨退变的关系。方法以2012年9月-2013年10月5例行关节镜下撕裂半月板部分切除患者自愿捐赠的半月板组织作为实验组,4例截肢患者自愿捐赠的正常半月板组织为对照组。取标本行HE染色,观察组织学改变;行实时荧光定量PCR,检测半月板中软骨退变相关基因[蛋白多糖(Aggrecan,ACAN)、Ⅹ型胶原(type X collagen,COL10A1)、基质金属蛋白酶13(matrix metalloproteinases 13,MMP-13)、CCAAT增强子结合蛋白β(CCAAT enhancer binding proteinβ,CEBP-β)、蛋白聚糖酶5(a disintegrin and metalloproteinase with thrombospondinmotif 5,ADAMTS-5)]以及mi RNAs(mi R-193b、mi R-92a、mi R-455-3p)表达水平。结果组织学观察示,实验组撕裂半月板组织存在不同程度退行性改变。与对照组相比,实验组ACAN表达水平下调,COL10A1、CEBP-β、ADAMTS-5、MMP-13表达水平均上调;除ACAN、MMP-13外,其余各退变相关基因组间差异均有统计学意义(P<0.05)。实验组mi R-193b、mi R-92a、mi R-455-3p表达水平均较对照组显著上调,比较差异亦有统计学意义(P<0.05)。结论撕裂半月板有退变趋势,其促进软骨退变作用较正常半月板显著,mi R-193b、mi R-92a、mi R-455-3p可能是促进软骨退变的调控因子。Objective To investigate the expressions of cartilage degenerative related genes in meniscus, and to evaluate the potential effect of meniscal damage on cartilage degeneration, and to analyze the relationship between microRNAs （miRNAs） expression and cartilage degeneration. Methods Meniscal tissue was collected from 5 patients undergoing partial meniscectomy between September 2012 and October 2013 （experimental group）, and normally meniscal tissue without tearing from amputees was used as controls （control group）. Pathological changes of menisci were observed; and real-time fluorescent quatitative PCR was performed to examine the relative expression levels of cartilage degenerative related genes and miRNAs： Aggrecan （ACAN）, type X collagen （COL10A1）, matrix metalloproteinases 13 （MMP-13）, CCAAT enhancer binding protein ~ （CEBP-~）, a disintegrin and metalloproteinase with thrombospondinmotif 5 （ADAMTS-5）, miR-193b, miR-92a, and miR-455-3p in meniscus. Results There were varying degrees of degenerative pathological changes in torn meniscus of experimental group. Compared with normal meniscus of control group, the expression of ACAN was decreased, while the expressions of COL10A1, CEBP-~, ADAMTS-5, and MMP-13 were increased in torn meniscus of experimental ACAN and MMP-13 （P〉0.05）. The expressions of miR-92a, group; and significant difference was found （P〈0.05） except miR-455-3p, and miR-193b in torn meniscus of experimental group were significantly higher than those in normal meniscus of control group （P〈0.05）. Conclusion Meniscal tissue has the intrinsic tendency of degeration after meniscus tear. The torn meniscus has greater stimulative impact on cartilage degeneration than normally morphological meniscus without tearing. The cartilage degenerative related miRNAs,including miR-193b, miR-92a, and miR-455-3p may contribute to the up-regulation of osteoarthritis.

关 键 词：半月板撕裂 骨关节炎 软骨退变基因 MIRNAS 

分 类 号：R684[医药卫生—骨科学]