针刺对肥胖大鼠血清瘦素及肝细胞JAK 2-STAT 3信号通路的影响  

Effect of Electroacupuncture Stimulation of“Housanli”(ST 36)and“Zhongwan”(CV 12)on Serum Leptin and Hepatocellular JAK 2-STAT 3 Signaling in Obese Rats

作　　者：严志康[1] 杨智杰[1] 陈锋[1] 

机构地区：[1]武汉大学人民医院针灸科,武汉430060

出　　处：《针刺研究》2015年第1期1-5,共5页Acupuncture Research

摘　　要：目的:观察手针和电针对肥胖大鼠血清瘦素、磷酸化的肝细胞Janus蛋白激酶2(p-JAK 2)、磷酸化的转录激活子3(p-STAT 3)蛋白的调节作用。方法:健康成年Wistar大鼠32只,随机分为正常组、模型组、手针组、电针组,每组8只。采用高脂饲料饮食造模,平均每只大鼠30g/d,连续喂养8周。造模成功后,手针组每日针刺大鼠"后三里"和"中脘"穴,电针组针刺并用电针刺激。治疗4周后检测各组大鼠体质量、体长、Lee’s指数,采用放射免疫法检测血清瘦素,Western blot法测定肝脏p-JAK 2、p-STAT 3蛋白水平。结果:模型组体质量、Lee’s指数、血清瘦素显著高于正常组(P<0.05),肝脏p-JAK 2、p-STAT 3显著低于正常组(P<0.05)。电针组和手针组体质量、Lee’s指数、血清瘦素显著低于模型组(P<0.05),肝脏p-JAK 2、p-STAT 3显著高于模型组(P<0.05)。手针组体质量、Lee’s指数、血清瘦素高于电针组(P<0.05),肝脏p-JAK 2、p-STAT 3低于电针组(P<0.05)。结论:电针和手针可以有效地降低肥胖大鼠的体质量、Lee’s指数,电针疗效优于手针。其作用机制可能与激活肝脏JAK 2-STAT 3信号通路,减轻肥胖大鼠瘦素抵抗有关。Objective To observe the effect of electroacupuncture（EA） stimulation of ＂Housanli＂ （ST 36） and ＂Zhong- wan＂ （OV 12） on serum leptin level and expression of phosphorylated Janus kinase 2 （p-JAK 2） and phosphorylated signal trans- ducer and activator of transcription 3 （p-STAT 3） proteins in the liver tissue of obese rats. Methods Thirty-two male Wistar rats were randomly divided into control group, model group, manual acupuncture （MA） group and EA group （n = 8 in each group）. The obese model was induced by high fat diet for 8 weeks. MA or EA （20 Hz, 1.5 V） stimulation was applied to bilateral ＂Housanli＂ （ST 36） and ＂Zhongwan＂ （OV 12） for 20 min, once daily for 4 weeks. The animals＇ body weight and length were re- corded, and Lee＇s index was calculated. Serum leptin level and liver p-JAK 2 and p-STAT 3 protein expression levels were de- tected using radioimmunoassay and Western blot, respectively. Results In comparison with the control group, the rats＇ body weight, Lee＇s index, and serum leptin content were remarkably increased in the model group （P〈0.05）, while hepatocellular p-JAK 2 and p-STAT 3 protein expression levels were significantly down-regulated in the model group （P〈0.05）. After treatment for 4 weeks, the levels of body weight, Lee＇s index, serum leptin were significantly reduced （P〈0.05）, and hepatocellular p-JAK 2 and p-STAT 3 protein expression in the MA and EA groups were considerably up-regulated compared with the model group （P〈0.05）. The effect of EA was apparently superior to that of MA in up-regulating p-JAK 2 and p-STAT 3 protein expression （ P〈0. 05）. Conclusion Both EA and MA stimulation can reduce the obese rats＇ body weight, and Lee＇s index, which may be closely associated with their effects in down-regulating serum leptin and in enhancing hepatocellular p-JAK 2 and p-STAT 3 protein expression.

关 键 词：电针 手针 肥胖 血清瘦素 肝脏JAK2-STAT 3信号通路 

分 类 号：R245.3[医药卫生—针灸推拿学]