方氏头针对血管性痴呆大鼠海马CA 1区星形胶质细胞凋亡的影响  

Effects of Scalp-acupuncture on Astrocyte Apoptosis in Hippocampal CA 1 Region in Rats with Vascular Dementia

作　　者：田文静[1] 黄琳娜 王瑞辉[3] 安军明 张萌[4] 

机构地区：[1]成都中医药大学针灸推拿学院,成都610000 [2]西安市中医医院针灸推拿科,西安710001 [3]陕西中医学院,咸阳712046 [4]河北北方学院中医学院,张家口075000

出　　处：《针刺研究》2015年第1期6-12,共7页Acupuncture Research

基　　金：陕西省自然科学基金(No.2012K190305)

摘　　要：目的:观察方氏头针对血管性痴呆大鼠海马CA 1区星形胶质细胞凋亡的影响,探讨方氏头针治疗血管性痴呆的机制。方法:健康雄性SD大鼠随机分为正常组、假手术组、模型组、方氏头针组,每组10只。采用改良Pulsinelli四血管阻断法制备血管性痴呆大鼠模型。方氏头针组针刺头部"倒脏上焦"(双侧)、"记忆"(双侧)、"思维"穴,每日1次。10d后,应用Morris智能水迷宫测试各组大鼠学习记忆能力的变化,采用免疫荧光双重标记技术检测各组大鼠海马CA 1区星形胶质细胞特异性标记物胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)与抑凋亡因子B细胞淋巴瘤/白血病-2(B cell lymphoma/leukemia-2,Bcl-2)共表达的阳性细胞数目,采用Western blot技术检测海马星形胶质细胞抑凋亡因子Bcl-2蛋白的表达。结果:水迷宫实验结果显示,平均逃避潜伏期及2min内首次跨越原象限平台的时间比较,模型组明显长于正常组及假手术组(P<0.01),方氏头针组明显短于模型组(P<0.01)。2min内跨越原象限平台次数比较,模型组明显少于正常组及假手术组(P<0.01),方氏头针组明显多于模型组(P<0.01)。与正常组及假手术组比较,模型组海马CA 1区GFAP/Bcl-2共表达的阳性细胞数明显降低,Bcl-2蛋白表达明显下降(P<0.01);与模型组比较,方氏头针组GFAP/Bcl-2共表达阳性细胞数显著增高,Bcl-2蛋白表达升高(P<0.01)。结论:方氏头针可显著上调血管性痴呆大鼠海马CA 1区星形胶质细胞抑凋亡因子Bcl-2的表达,从而有效改善血管性痴呆大鼠的学习记忆能力。Objective To observe the effect of scalp-acupuncture intervention on the expression of Bcl-2 of astrocytes in the hippocampus in vascular dementia （VD） rats,so as to reveal its underlying mechanism in improving VD. Methods A total of 40 male SD rats were randomly divided into normal control, sham-operation, VD model, and scalp-acupuncture groups （n = 10 in each group）. The VD model was established by using modified Pulsinelli 4-vessel-occlusion method. Scalp-acupuncture was ap- plied to bilateral ＂Daozang Shangjiao＂ and bilateral ＂Jiyi＇, and ＂Siwei＂ acupoints according to FANG＇s scalp-acupuncture therapy for 30 min, once daily for 10 days. The rat^s learning-memory ability was detected by Morris water maze tests, and the expres- sion of apoptosis related proteins Bcl-2 of astrocytes in the hippocampus was detected by immunefluorescence double labeling technique and Western blot, respectively. Results Morris water maze tests showed that the average escape latency of place navigation test and the duration of the first platform-site crossover （space probing test） were significantly increased in the model group compared with the normal control group （P〈0.01）, and the number of platform-site crossovers in 2 min was markedly de-creased in the model group （P〈0.01）. The increased average escape latency and the increased duration of first platform-site crossover were markedly shortened in the scalp-acupuncture group in comparison with the model group （P〈0.01）, and the num- ber of the platform crossovers in 2 min was significantly increased in the scalp-acupuncture group（P〈0.01）. The expression of Bcl-2 protein and the number of GFAP/Bcl-2-1abeled cells of hippocampal CA 1 region in the model group were remarkably de- creased compared with the normal control group （P〈0.01）. After scalp-acupuncture intervention, the decreased Bcl-2 protein expression level and the number of double-labeled GFAP/Bcl-2 cells were significantly up-regulated in the scalp-acupuncture group c

关 键 词：头针干预 血管性痴呆 学习记忆能力 海马 CA 1区 星形胶质细胞 BCL-2蛋白 GFAP/Bcl-2双标 

分 类 号：R245.321[医药卫生—针灸推拿学]