南京市成人星状病毒性腹泻病原基因特征分析  

Genetic characteristics of astrovirus in adult outpatients with acute gastroenteritis, Nanjing

作　　者：王炜[1] 何敏[1] 石利民[1] 王璇[1] 乔梦凯[1] 张洪英[1] 

机构地区：[1]南京市疾病预防控制中心,江苏南京210003

出　　处：《现代预防医学》2015年第7期1284-1285,1289,共3页Modern Preventive Medicine

基　　金：南京市医学科技发展计划项目(ZKX09033);南京市卫生青年培养工程项目(QRX11127)

摘　　要：目的对2013年南京市疑似病毒性腹泻成人门诊样本进行星状病毒核酸检测、分子分型和进化分析,为星状病毒所致成人病毒性腹泻的分子流行病学提供资料。方法收集2013年疑似病毒性腹泻门诊成人病例的腹泻物375份,用星状病毒特异性引物和探针进行荧光定量RT-PCR检测,然后对荧光定量RT-PCR阳性的样本,用一步法RT-PCR再进行星状病毒特异片段的检测。对RT-PCR产物进行双向测序和进化树分析。结果荧光定量RT-PCR共检测到9例星状病毒阳性(2.4%),其中,6例(1.6%)扩增到单一条带,测序获得449 bp的星状病毒壳蛋白基因片段序列。经比对分析,6例星状病毒中,4例为5型星状病毒,1例为1型星状病毒,1例为8型星状病毒。结论南京市星状病毒所致成人病毒性腹泻中有多种型别星状病毒,5型为优势型。Objective To provide data on the molecular epidemiology of astrovirus in adult outpatients with suspected viral diarrhea by nucleic acid detection, genotyping and evolution analysis. Methods 375 suspected cases of viral diarrhea were collected in 2013,which were fluorescent quantitative RT-PCR tested by with specific primers and probe for astrovirus. The positive cases were then tested again by one-step RT-PCR method for specific nucleic acid fragments. PCR products were bidirectionally sequenced and phylogenetically analyzed. Results There were nine（2.4%） positive cases in all samples, of which six cases amplified a single band and totally obtained 449 bp of astrovirus capsid protein gene sequence fragments. From the comparative analysis, there were four cases of type 5 astrovirus, one case of type 1 astrovirus, and one case of type 8 astrovirus in the six cases of astrovirus. Conclusion Viral diarrheas in adults were caused by a variety of type＇s astrovirus in Nanjing and type 5 astrovirus was the dominant type.

关 键 词：腹泻 病原学 星状病毒 荧光定量RT-PCR RT-PCR 

分 类 号：R115[医药卫生—公共卫生与预防医学]