留置针无针接口微生物屏障能力评价方法  

Eevaluation method on the microbial barrier ability for remaining needlele connector

作　　者：方菁嶷[1] 邹婷[2] 徐琳[2] 宋宏晖[3] 

机构地区：[1]苏州大学医学部,江苏苏州215123 [2]苏州大学卫生与环境技术研究所 [3]苏州大学附属第二医院

出　　处：《现代预防医学》2015年第7期1290-1292,1305,共4页Modern Preventive Medicine

摘　　要：目的评价静脉留置针无针接头部分的微生物屏蔽能力。方法以金黄色葡萄球菌(ATCC 6538)、表皮葡萄球菌(ATCC 12228)、大肠杆菌(ATCC 8739)和铜绿假单胞菌(ATCC 9027)为指示微生物,模拟临床环境及操作,染菌后回收活菌并计数。结果菌悬液浓度,验证组及对照组活菌回收数说明实验过程符合设计要求;结果显示,染菌后四种菌种d 1活菌回收数均为0,随着时间的推移活菌回收量呈线性趋势增加,第五天金黄色葡萄球菌、表皮葡萄球菌、大肠杆菌和铜绿假单胞菌回收均数分别达到737,935,1238,453 cfu/件。结论时间越长,无针接头部分的微生物屏障能力越差;无针接口的材料有待改进;不得已用留置针的情况下、尽量缩短使用周期。Objective To evaluate the remaining microbial barrier ability of the venous retention needle for needlele connector.Methods We used Staphylococcus aureus（ATCC 6538）, Staphylococcus epidermidis（ATCC 12228）, Escherichia coli（ATCC 8739）and Pseudomonas aeruginosa（ATCC 9027） as biological indicators, to imitate the clinical environment and operation. Then we got the viable count after inoculation. Results The concentration of bacterial suspension, viable counts of the verification and control groups all met the study design; The viable counts after inoculation of the test groups linear increased from 0 to about 737 cfu/device（Staphylococcus aureus）, 935 cfu/device（Staphylococcus epidermidis）, 1238 cfu/device（Escherichia coli） and 453 cfu/device（Pseudomonas aeruginosa） respectively on the fifth day. Conclusion The microbial barrier ability of needlele connector gets worse as time goes on. Improving the material and shortening the service cycle is necessary for the use of venous retention needle.

关 键 词：留置针 无针接口 微生物屏障能力 

分 类 号：R118[医药卫生—公共卫生与预防医学]