pH-induced structural changes of surface immobilized poly (L-lysine) by two-dimensional (2D) infrared correlation study  

作　　者：Eun Joo Yoo Boknam Chae Young Mee Jung Seung Woo Lee 

机构地区：[1]School of Chemical Engineering,Yeungnam University [2]Pohang Accelerator Laboratory [3]Department of Chemistry,Kangwon National University

出　　处：《Chinese Chemical Letters》2015年第2期173-176,共4页中国化学快报（英文版）

基　　金：supported by Yeungnam University Research Grants in 2013;a Human Resources Development Program of Korea Institute of Energy Technology Evaluation and Planning (KETEP) grant(No.20104010100580) funded by the Korean Ministry of Knowledge Economy

摘　　要：This paper reports the pH-induced structural changes in the surface immobilized poly（L-lysine）（PLL）film.Two-dimensional（2D） correlation analysis was applied to the Fourier transform infrared（FTIR）spectra of the surface-immobilized PLL film to examine the spectral changes induced by the alternations of the protonation state of the amino group in the side chain.Significant spectral changes in the FTIR spectra of the PLL film were observed between pH 7 and 8.The decrease in the protonation state of the amino group in the side chain induced spectral changes in the amino group as well as conformational changes in the alky]group in the side chain.From pH 1-8,the spectral changes in the amino and alkyl groups in the side chain occurred before those of the amide group in the main chain of the surface immobilized PLL film.This paper reports the pH-induced structural changes in the surface immobilized poly（L-lysine）（PLL）film.Two-dimensional（2D） correlation analysis was applied to the Fourier transform infrared（FTIR）spectra of the surface-immobilized PLL film to examine the spectral changes induced by the alternations of the protonation state of the amino group in the side chain.Significant spectral changes in the FTIR spectra of the PLL film were observed between pH 7 and 8.The decrease in the protonation state of the amino group in the side chain induced spectral changes in the amino group as well as conformational changes in the alky]group in the side chain.From pH 1-8,the spectral changes in the amino and alkyl groups in the side chain occurred before those of the amide group in the main chain of the surface immobilized PLL film.

关 键 词：Poly（L-lysine）（PLL） Surface-immobilized polypeptide FT-IR spectroscopy 2D correlation spectroscopy 

分 类 号：O631.11[理学—高分子化学]