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机构地区:[1]上海第二工业大学环境与材料工程学院,上海201209 [2]常州纺织服装职业技术学院纺织化学工程系,江苏常州213000
出 处:《上海第二工业大学学报》2015年第1期7-11,共5页Journal of Shanghai Polytechnic University
基 金:上海高校青年教师培养资助计划(No.ZZegd14014);上海第二工业大学校级科研项目(No.EGD13XQD19);上海第二工业大学"环境工程"校培育学科项目(No.XXKPY1303)资助
摘 要:通过控制直流电场强度对氧化亚铁硫杆菌(Thiobacillus ferrooxidans,T.f.)进行电场刺激实验,探究直流电场电动力学效应对T.f.生物活性的影响以及繁殖代谢情况。考察分析了不同弱电流强度(100 m A)下,T.f.的菌密度、菌液p H变化、菌液总蛋白量以及细菌形态的变化。实验结果表明,与未施加电场的对照组对比,在20 m A电流强度作用下,明显有增加T.f.的菌密度的作用,过高的电流抑制了细菌的生长。通过实验发现当施加20 m A电流时,氧化亚铁硫杆菌胞内蛋白总量达到未施加电场的2.15倍,能够明显提高T.f.细胞膜的通透性,增加细胞膜的厚度。这对于大规模化利用T.f.浸出废弃线路板中金属提供了科学依据和参考。The direct current intensity affect on Thiobacillus ferrooxidans(T.f.) were controlled, for purpose of elcctric field stimulation experiments. Effect of direct current field electrodynamics on T.f. and metabolism of them were explored. Such as, density of T.f, p H change and protein content of microbial, and changes in the form of bacteria were investigated under low current intensity( 100 m A).The experimental results showed that under the appropriate electric fields for instance 20 m A have positively effects on maintaining the density of T.f., and high density of current inhibited the growth of bacteria, compared with the control group of not applying an electric field. It was also found by experiments that T.f. cell membrane permeability were increased and the thickness of the membrane obviously amounted to 2.15 times that no applying electric field under the effect of 20 m A current strength. The results provided scientific basis and reference for the large scale using T.f. bioleaching waste printed circuit boards.
分 类 号:TN41[电子电信—微电子学与固体电子学]
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