三聚氰胺联合三聚氰酸对攻毒大肠杆菌小鼠抵抗力的影响  

Effect of Melamine and its Combination with Cyanuric Acid on the E.coli Resistance of Mice

作　　者：王欣[1] 李化生[1] 王文超[1] 刘娇[1] 王心竹[2] 尹荣兰[3] 李曦婷[1] 董俏 田菁菁 尹荣焕[1] 

机构地区：[1]沈阳农业大学畜牧兽医学院,辽宁沈阳110161 [2]辽宁农业职业技术学院畜牧兽医系,辽宁营口115009 [3]吉林省畜牧兽医科学研究院,吉林长春130062

出　　处：《家畜生态学报》2015年第2期67-71,共5页Journal of Domestic Animal Ecology

基　　金：国家自然科学基金(31001088);辽宁省教育厅"优秀人才"支持项目(LJQ2013070);沈阳农业大学"天柱山英才"项目(2012-4)

摘　　要：试验旨在研究三聚氰胺及其同系物对机体免疫力产生的可能影响,为完善该类污染物的毒理学评价资料提供依据。试验将雄性KM小鼠144只随机分为8组,分别为空白对照组、大肠杆菌阳性对照组,试验组包括三聚氰胺高、中、低剂量组(50 mg/kg、10 mg/kg、2mg/kg)、三聚氰胺与三聚氰酸联合高、中、低(25+25mg/kg、5+5mg/kg、1+1mg/kg)剂量组,试验组隔天灌胃15d后,腹腔注射大肠杆菌,48h后检测小鼠脾脏B淋巴细胞增殖率、血清溶血素光密度值和迟发性变态免疫反应指标。研究结果表明,与对照组相比,当三聚氰胺和三聚氰酸攻毒剂量达到25+25mg/kg时,B淋巴细胞转化能力下降(0.01<P<0.05),血清溶血素吸光度值下降(P<0.01),当攻毒剂量达到三聚氰胺50mg/kg、三聚氰胺和三聚氰酸剂量25+25mg/kg、5+5mg/kg时,小鼠足趾增厚度减小(P<0.01),提示三聚氰胺和三聚氰酸能够降低小鼠对大肠杆菌的抵抗能力。To test the possible effect of melamine and its homologues on the immunity of organism and provide reliable data for supplementing the toxicological evaluation data of such pollutants,the present study divided male KM mice into eight groups：the control group,E.coli positive control group,melamine high（50mg/kg）,medium（10mg/kg）and low（2mg/kg）dose groups,melamine and cyanuric acid high（25＋25mg/kg）,medium（5＋5mg/kg）and low（1＋1mg/kg）dose groups.The test groups were fed every other day for 15 days and detected the spleen B lymphocyte transformation rate,hemolysin index optical density,etc.48 hours after the intraperitoneal injection with E.coli thereafter.The results showed that compared with the control group,when the agents of melamine and cyanuric acid reached 25＋25mg/kg,B lymphocyte transformation capacity（0.01〈P〈0.05）and hemolysin absorbance values（P〈0.01）decreased;when the dose of melamine reached 10mg/kg,melamine 50mg/kg,and the combinational dose of melamine and cyanuric acid reached 25＋25mg/kg,thickness growth of mice toe decreased.The results suggested that melamine and cyanuric acid can reduce the E.coli resistance of mice.

关 键 词：三聚氰胺 三聚氰酸 淋巴细胞转化能力 血清溶血素 抵抗力 

分 类 号：S811.6[农业科学—畜牧学]