2-BFI对星形胶质细胞糖氧剥夺损伤的保护及抗线粒体凋亡的研究  

The Protective effect and resistance mitochondrial apoptosis of 2-BFI on oxygen-glucose deprivation of cultured rat cortical astrocytes

作　　者：田继沙[1] 杨金龙[2] 程朝辉[2] 刘双[2] 韩钊[3] 

机构地区：[1]江苏省淮安市第二人民医院神经内科,223000 [2]浙江省温州医科大学附属第一医院脑血管科,325000 [3]浙江省温州医科大学附属第二医院神经科,325000

出　　处：《中华全科医学》2015年第4期565-567,F0003,共4页Chinese Journal of General Practice

摘　　要：目的探讨2-BFI对星形胶质细胞糖氧剥夺损伤(oxygen-glucose deprivation,OGD)的保护作用,并从抗线粒体凋亡途径探讨2-BFI抗糖氧剥夺损伤的机制。方法原代培养星形胶质细胞,制成糖氧剥夺损伤模型,分为对照组、OGD组、OGD+2-BFI组,药物组分为0.75、1.5、3.0、6.0、12.0(μg/ml)五个浓度,2-BFI干预后测定细胞活力,选出最佳的药物浓度,以该浓度进一步在细胞水平上研究2-BFI对线粒体膜电位、caspase-3活性、细胞凋亡率的影响。组间比较采用SPSS 17.0进行单因素方差分析。结果各个浓度的2-BFI均能提高糖氧剥夺损伤后的星形胶质细胞存活率,其中以1.5μg/ml的效果最佳;给予浓度为1.5μg/ml 2-BFI干预OGD组能显著提高线粒体膜电位(13.50±5.88,8.48±4.79,P<0.05),抑制caspase-3活性(4.56±0.10,4.85±0.10,P<0.05)、降低细胞凋亡率[(24.6±1.60)%,(46.5±1.10)%,P<0.05]。结论星形胶质细胞糖氧剥夺损伤后,2-BFI具有提高细胞存活率,稳定线粒体膜电位、抑制caspase-3活性,进而降低细胞凋亡率的作用。Objective To explore the neuroprotective effects and the resistance mitoehondrial apoptosis pathway of 2-BFI on oxygen-glucose deprivation of astrocytes. Methods Making into the model of oxygen-glucose deprivation of astrocytes. They were designed into three groups： normal control group, OGD group and OGD ± 2-BFI group,the concentration of drug were 0. 75,1.5,3.0,6. 0, 12.0 （ μg/ml）, to determine the cell ability of astrocytes, choose the best drug concentration,then use the best drug density to measure mitochondrial membrane potential（△ψm） ,caspase-3 activity and Hoechst 33 258 staining. Comparison between groups using SPSS17. 0 to single factor analysis of variance was performed. Results Different concentration of 2-BFI can significantly raise the cell ability, and the effect of 1. 5μg/ml was the best;1.5 μg/ml 2-BFI group can significantly increase △ψm（ 13.50 ±5.88,8.48 ±4.79, P 〈0.05） ,inhibiting the ac- tivation of caspase-3（4.56 ±0.10,4.85 ±0.10, P 〈0.05） ,further inhibit the apoptosis[ （24.6 ± 1.6）%, （46.5 ± 1.1 ） % ,P 〈 0. 05） ]. Conclusion When the cortical astrocytes were deprived of oxygen and glucose,2-BFI could effectively raise the cell ability, stabilize Am and inhibit the activation of caspase-3, further inhibit the apoptosis.

关 键 词：咪唑啉受体 2-BFI 凋亡 线粒体膜电位 caspase-3 

分 类 号：R929.25[医药卫生—药学] R743.3