哮喘患儿ORMDL3基因多态性与溶血卵磷脂及载脂蛋白B的关系  

Association of ORMDL3 single nucleotide polymorphisms with lysophosphatidylcholine and apolipoprotein B levels in children with asthma

作　　者：王冰洁[1] 王桂兰[1] 陈德晖[2] 王文祥[3] 黄娟[1] 容嘉妍[1] 刘翔腾[1] 杨赛[1] 

机构地区：[1]南方医科大学附属中山博爱医院小儿呼吸科,广东中山528400 [2]广州医科大学附属第一医院儿科,广东广州510000 [3]南方医科大学附属中山小榄人民医院儿科,广东中山528400

出　　处：《中国当代儿科杂志》2015年第3期241-244,共4页Chinese Journal of Contemporary Pediatrics

摘　　要：目的探讨哮喘患儿ORMDL3基因多态性与溶血卵磷脂(Lyso-PC)及载脂蛋白B(apo B)的关系。方法选取2010年1月至2012年12月诊断为支气管哮喘的300例患儿为哮喘组,另选取同期诊断为上呼吸道感染的298例患儿为对照组。ELISA法检测两组患儿血清中Lyso-PC和apo B水平;Taq Man探针法进行基因型分析。结果哮喘组Lyso-PC、apo B水平明显高于对照组(P<0.01);哮喘组ORMDL3基因rs12603332位点不同基因型患儿Lyso-PC、apo B水平亦显著高于对照组(P<0.01);CC基因型哮喘患儿Lyso-PC、apo B水平明显高于CT和TT基因型患儿(P<0.01)。结论 Lyso-PC及apo B可能干预哮喘发生的病理过程;血清中高Lyso-PC、apo B水平可能与哮喘易感基因ORMDL3多态性rs12603332位点共同作用导致儿童哮喘的发生。Objective To study the association of ORMDL3 single nucleotide polymorphisms （SNP） with lysophosphatidylcholine （LysoPC） and apolipoprotein B （apoB） levels. Methods A total of 300 children diagnosed with bronchial asthma between January 2010 and December 2012 were selected for the asthma group, and 298 children diagnosed with upper respiratory tract infection in the same period were selected for the control group. Serum LysoPC and apoB levels were measured using enzyme-linked immunosorbem assay. Genotype analysis was performed using the TaqMan probe. Results LysoPC and apoB levels were significantly higher in the asthma group than in the control group （P〈0.01）. Among children with various genotypes of ORMDL3 gene at locus rs12603332, the asthma group had significantly higher LysoPC and apoB levels than the control group （P〈0.01）. Among the children with asthma, those with CC genotype had significantly higher LysoPC and apoB levels than those with CT and TT genotypes （P〈0.01）. Conclusions LysoPC and apoB may intervene in the pathological process of asthma. Pro-inflammatory gene ORMDL3 SNP rs12603332 may be associated with high LysoPC and apoB levels, which leads to the occurrence of childhoodasthma.

关 键 词：ORMDL3基因 基因多态性 溶血卵磷脂 载脂蛋白B 哮喘 儿童 

分 类 号：R725.6[医药卫生—儿科]