儿童传染性单核细胞增多症血浆蛋白质组学分析  

Plasma proteomic analysis in children with infectious mononucleosis

作　　者：冉志玲[1] 肖斌[2] 刘红瑞[1] 刘友平[2] 盛巧妮[1] 

机构地区：[1]泸州医学院附属中医院儿科,四川泸州646000 [2]泸州医学院生物化学与分子生物学教研室,四川泸州646000

出　　处：《中国当代儿科杂志》2015年第3期254-257,共4页Chinese Journal of Contemporary Pediatrics

基　　金：泸州市科技计划项目(2012-S-38)

摘　　要：目的通过对比分析传染性单核细胞增多症(infectious mononucleosis,IM)患儿与同龄健康儿童(对照组)的血浆蛋白质组图谱的差异表达,初步探索IM特异表达或异常表达的血浆"分子标志物"以协助临床诊断。方法采用双向电泳技术(2-DE)结合质谱技术对IM患儿与对照组的血浆全蛋白进行比较蛋白质组学分析。结果比较蛋白质组学分析发现IM患儿组与对照组比较具有明显差异表达蛋白质共有7个:血色素结合蛋白(Hpx)、Vit D3结合蛋白(DBP)、胎球蛋白A(fetuin A)、C-反应蛋白(CRP)、载脂蛋白A(Apo A)、结合珠蛋白(HPT)、甲状腺运载蛋白(TTR),其中,HPT表达量较对照组明显升高,其余蛋白的表达量均较对照组明显降低。结论 IM患儿与对照组比较存在明显差异表达的蛋白质,这些差异蛋白表达量的改变均与肝脏的急性损伤有关,提示儿童IM常伴有肝脏的急性损伤。若对这些差异表达的蛋白进一步分析,可望能够找到为IM临床诊断和治疗提供一定参考价值的血浆特异性标志物。Objective To explore the abnormal expression of plasma proteins by analysis ofproteomic expression profile in children with infectious mononucleosis （IM）. Methods Two dimensional gel electrophoresis （2-DE） followed by the mass spectrometry was used to examine important protein spots with different expression levels between children with IM and normal controls. Results Seven differential proteins were obtained： hemopexin, vitamin D binding protein, fetuin A, C-reactive protein, apolipoprotein A, haptoglobin and transthyretin. Compared with the control group, haptoglobin showed a higher expression level in children with IM, and the expression levels of the other proteins were obviously down-regulated. Conclusions The expression changes of differential proteins identified in this study are all related with the liver acute injury, suggesting that children with IM are associated with acute liver injury. Further studies on the characteristics of above proteins will contribute to the diamaosis and treatment ofoediatric IM.

关 键 词：传染性单核细胞增多症 血浆蛋白 蛋白质组学 儿童 

分 类 号：R725.1[医药卫生—儿科]