db/db小鼠心、肾RAS组分的分子表达  

Molecular expression of RAS components in heart and kidney of db / db mice

作　　者：杨久琳 刁腾月 张岩[1] 

机构地区：[1]上海理工大学系统生物医学研究中心,上海200093

出　　处：《基础医学与临床》2015年第3期341-344,共4页Basic and Clinical Medicine

基　　金：上海市科委资助课题-浦江人才计划(10PJ1407700)

摘　　要：目的探讨2型糖尿病诱导心脏、肾脏肾素-血管紧张素系统(RAS)组分的表达变化。方法用db/db小鼠作为2型糖尿病动物模型,RT-PCR和蛋白免疫印迹法分别检测心脏、肾脏中RAS组分的基因和蛋白表达。结果 db/db小鼠心脏AGT mRNA表达、renin和AngⅡ蛋白表达显著升高(P<0.01);而肾脏renin mRNA(P<0.001)和蛋白(P<0.01)表达以及AngⅡ(P<0.05)的蛋白表达显著上调。结论 2型糖尿病诱导心脏、肾脏RAS组分的异常表达,最终引起RAS活性肽AngⅡ生成增加。Objective To investigate the type 2 diabetes-induced expression changes of renin-angiotensin system（ RAS） components in heart and kidney. Methods The db /db mice were applied to be the type 2 diabetes animal model. Gene and protein expression of RAS components in heart and kidney were measured by RT-PCR and immunoblotting,respectively. Results The mRNA expression of AGT（ P 0. 01） and the protein expression of renin（ P 0. 01） and Ang Ⅱ（ P 0. 01） were significantly increased in heart of db / db mice,moreover,the mRNA（ P 0. 001） and protein（ P 0. 01） expression of renin and the protein expression of Ang Ⅱ（ P 0. 05） were significantly up-regulated in kidney of db / db mice. Conclusions Type 2 diabetes induces abnormal expression of RAS components in heart and kidney and consequently leading to the increase of production of RAS active peptide Ang Ⅱ.

关 键 词：肾素-血管紧张素系统 心脏 肾脏 2型糖尿病 

分 类 号：R587.1[医药卫生—内分泌]