砷暴露对子代小鼠肺组织ERα表达性别差异的初步研究  

Gender-dependent Expression of ERαin Arsenic Exposed Mice Offspring's Lung Tissue

作　　者：石萌萌[1] 陈国建[1] 张洋[1] 车望军[2] 张浩[1] 

机构地区：[1]四川大学华西公共卫生学院环境卫生与职业医学系,成都610041 [2]昆明市疾病预防控制中心,昆明650022

出　　处：《四川大学学报（医学版）》2015年第2期197-200,共4页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(No.81160340)资助

摘　　要：目的观察亲代母鼠砷染毒后子代不同发育时期肺组织内雌激素受体α(ERα)的表达变化规律及性别间的差异,探讨砷暴露在肺癌发生性别差异中的作用。方法亲代母鼠在孕第8d至子代断乳期砷灌胃染毒,子代在断乳期后砷饮水染毒至成年期。通过实时荧光定量PCR和Western blot技术检测子代雌雄小鼠在不同发育时期、不同剂量(低、中、高)亚砷酸钠染毒情况下,肺组织中ERαmRNA和蛋白的表达水平。结果胚胎期雌性肺组织中ERαmRNA表达水平低于雄性(P<0.05);断乳期和成年期雌性肺组织中ERαmRNA表达水平高于雄性(断乳期中剂量组P<0.05,成年期中、高剂量组P<0.05);胚胎期、断乳期、成年期各期对照组肺组织内ERαmRNA的表达水平差异无统计学意义;断乳、成年期雌性肺组织内ERαmRNA的表达水平均高于胚胎期雌性(低、中、高剂量组P<0.05)。断乳期和成年期雌性各染毒剂量组肺组织中ERα蛋白表达水平均高于雄性,与对照组比较亦增高(P<0.05)。成年期子代雌性、雄性小鼠各砷染毒组肺组织ERα蛋白的表达水平均高于断乳期(P<0.05)。结论亲代母鼠砷染毒可使子代雌性小鼠在断乳期、成年期时肺组织内ERα表达水平均高于雄性小鼠,对阐明环境污染物在肺癌发生性别差异中的作用具有重要的意义。Objective To observe theexpression variations of estrogen receptor alpha（ERα）in different periods of mice offspring and the gender-dependent differences in the lung tissue with parental arsenic exposure.Methods Parental female mice were exposed to arsenic by gavage from gestational day 8th to offspring infancy,and offspring were exposed to arsenic by drinking water after infancy.The expression level of ERαmRNA and protein in lung tissue of the male and female offspring in different developmental periods and different doses（low,middle,high）of sodium arsenite exposure were detected by real-time PCR and Western blot.Results ERα mRNA expression in female lung tissue was lower than male in embryonic period（P〈0.05）;ERα mRNA expression in female lung tissue was higher than that of male in infant and adult periods（middle dose of infancy P〈0.05,middle and high doses of adulthood P〈0.05）;No statistical significances were observed in embryo,infancy and adulthood control groups.ERα mRNA expression in female lung tissue of infancy and adulthood was higher than that in embryonic period（low,middle and high dose groups P〈0.05）.ERαprotein expression in arsenic exposed female lung tissue was higher than that of male in infant and adult periods,it was also increased by compared with corresponding control groups（P〈0.05）.The expression level of ERαprotein in exposed adult female and male offspring were higher than that of infancy.Conclusion Arsenic infected during pregnancy can increase the lung tissue＇s ERαexpression level of female offspring in infancy and adulthood.This result is significant to elucidate the role of environment pollutants in gender difference of lung cancer＇s occurrence.

关 键 词：雌激素受体Α 肺组织 亚砷酸钠 小鼠 

分 类 号：R114[医药卫生—卫生毒理学]