雌激素快速诱导小鼠囊胚细胞中钙离子增加在囊胚着床中的作用  

Estrogen Induced Rapid Increase of Intracellular Calcium Plays Important Role in the Implantation of Mouse Blastocysts

作　　者：张诗茂 喻琳麟[1,2] 曲婷[1] 袁东智[1] 张金虎[1] 何亚平[1] 岳利民[1] 

机构地区：[1]四川大学华西基础医学与法医学院生理学教研室,成都610041 [2]成都市计划生育指导所,成都610031

出　　处：《四川大学学报（医学版）》2015年第2期205-208,共4页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(No.31100844)资助

摘　　要：目的观察雌激素快速诱导小鼠囊胚细胞中钙离子增加在囊胚着床中的作用。方法获取小鼠囊胚,将其分为空白组、耦联牛血清白蛋白的大分子雌激素组(E2-BSA组)和钙离子螯合剂预处理再给予E2-BSA组(BAPTA+E2-BSA组),采用免疫细胞化学染色结合共聚焦显微镜观察,囊胚与子宫内膜上皮细胞体外共培养和异体胚胎移植技术研究雌激素快速诱导的小鼠囊胚细胞中钙离子增加对囊胚细胞整合素的表达和定位以及对囊胚黏附和植入的影响。结果共聚焦显微镜观察结果显示E2-BSA可引起囊胚细胞整合素αv、β3的聚集,而BAPTA可阻断雌激素所引起的整合素的聚集和迁移。体外黏附实验结果显示,空白对照组和BAPTA+E2-BSA组的囊胚黏附率分别为35.5%和26.7%,低于E2-BSA组的囊胚黏附率(65.6%,P<0.05)。体内移植实验结果显示,BAPTA+E2-BSA组的囊胚着床率11.8%低于E2-BSA组(52.9%,P<0.05)。结论雌激素诱导的小鼠囊胚细胞内钙离子的快速增加可引起整合素聚集,并在囊胚的黏附和植入过程中发挥重要作用。Objective To study the roles of the increased intracellular calcium induced rapidly by estrogen in the implantation of mouse blastocysts.Methods The mouse blastocysts were collected from the female mice on the pregnant day 4,divided into 3groups：control,E2-BSA and BAPTA＋E2-BSA.Immunofluorescence staining,confocal microscopy,embryo and endometrial epithenial cells co-culture and embryo transfer were used to investigate the effect of increased intracellular calcium induced by E2-BSA on the expression and localization of integrins in blastocysts and their adhesion to endometrial epithenial calls（EECs）and implantation into the endometrium.Results The increase of intracellular calcium induced rapidly by estrogen could cause the cluster and relocation of integrinαv andβ3,and BAPTA might block this effect,the adhesion rate of blastocysts in contol group was 35.5%,BAPTA＋E2-BSA group was 26.7%and significantly lower than 65.6% of E2-BSA group（P〈0.05）,and the implantation rate in BAPTA＋E2-BSA group was 11.8%,which was significantlly lower than 52.9% of E2-BSA group（P〈0.05）.Conclusion The rapid increase of intracellular calcium induced by estrogen may cause the relocalization of integrin in blastocysts and their adhesion to ECCs,which is important in the process of implantation.

关 键 词：雌激素 钙离子 整合素 囊胚 着床 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学]