酶催化光度法测定药物中的双嘧达莫  

Determination of dipyridamole by enzymatic catalytic spectrophotometry in pharmaceuticals

作　　者：王帆[1] 梅玉强 朱文平[1,3] 陈超华[1] 陈亚红[1,3] 

机构地区：[1]周口师范学院化学化工学院,河南周口466001 [2]周口市质量技术监督检验测试中心,河南周口466000 [3]周口师范学院药物化学研究所,河南周口466001

出　　处：《周口师范学院学报》2015年第2期71-73,共3页Journal of Zhoukou Normal University

基　　金：河南省科技厅基础与前沿技术研究项目(No.142102310365);河南省教育厅科学技术重点项目(No.14A150015);周口师范学院科研创新基金资助(No.zknuA201409)

摘　　要：基于在pH 9.8的NH3-NH4Cl的缓冲溶液中,双嘧达莫对血红蛋白酶催化体系有较强的抑制作用,建立了测定双嘧达莫的酶催化光度分析新方法.当双嘧达莫的质量浓度在1.2-29.2μg/mL内与其抑制程度有良好的线性关系,检出限为0.06μg/mL.双嘧达莫的质量浓度为3.5μg/mL时,11次平行测定的相对标准偏差为2.3%.该方法简单、快速,可用于双嘧达莫片中和注射液中双嘧达莫含量的测定.A simple spectrophotometric method for the determination of dipyridamole based on the inhibition effect of hemoglobin was developed in the NH3·H2O-NH4 Cl buffer.The percentage inhibition of system is calculated under the optimal experimental conditions.The calibration curve is linear in the range of 1.2-29.2μg/mL,with the detection limit of 0.06μg/mL.The relative standard deviation of this method is 2.3%at 3.5μg/mL for 11 determination.This method is simple and rapid which is suitable for the determination of dipyridamole in pharmaceuticals with satisfactory results.

关 键 词：双嘧达莫 酶催化光度法 血红蛋白 

分 类 号：O657.2[理学—分析化学]