荧光光谱法研究磺脲类降糖药物与牛血清白蛋白的相互作用  

Fluorescence spectrometric studies on interaction of bovine serum albumin with sulfonylurea drugs

作　　者：刘家琴[1] 王坤[1] 闻春雷 李美洁[1] 

机构地区：[1]西华大学物理与化学学院,四川成都610039

出　　处：《周口师范学院学报》2015年第2期79-82,共4页Journal of Zhoukou Normal University

基　　金：四川省大学生创新创业训练计划项目(No.201310623035);西华大学重点项目(No.Z0913305)

摘　　要：用荧光光谱法研究了模拟生理条件下磺脲类降糖药物格列美脲、格列本脲与牛血清白蛋白(BSA)的相互作用.结果表明:格列美脲、格列本脲均对BSA的荧光有猝灭作用,其猝灭机理均为静态猝灭.根据不同温度(298/297,302,310K)下格列美脲、格列本脲与BSA作用的荧光强度,由修正的Stern-Volmer方程计算了二者与BSA反应的结合常数,其值分别为2.239×105,1.857×105,1.247×105 L·mol-1和1.549×105,0.825 2×105,0.386 5×105 L·mol-1.根据van’t Hoff方程计算了反应的热力学参数,并讨论了格列美脲、格列本脲与BSA的主要作用力类型,其结果表明二者与BSA间的作用主要为氢键和范德华力.The interactions of glimepiride/glibenclamide and bovine serum albumin（BSA）in physiological conditions solution（Tris-HCl buffer,pH 7.40）have been studied by fluorescence spectrometry.The results showed that the fluorescence intensity of BSA at 343 nm was quenched intensively by glimepiride/glibenclamide.According to the quenching effects of glimepiride/glibenclamide on the fluorescence intensity of BSA under different temperatures,and the quenching mechanisms were confirmed to be a static one.The binding constants and thermodynamic parameters were calculated.In the meantime,the main acting force between glimepiride/glibenclamide and bovine serum albumin（BSA）was discussed.The results showed the interaction force between glimepiride/glibenclamide and BSA belongs to hydrogen bond and Van der Waals force.

关 键 词：格列美脲 格列本脲 牛血清白蛋白 相互作用 荧光光谱法 

分 类 号：O657.2[理学—分析化学]