血浆替代血清行常规生化检测缩短正流转时间的可行性探讨  

Feasibility of using heparinized plasma instead of serum sample for conducting routine biochemical tests to shorten TAT

作　　者：黄学梅[1] 喻垚[1] 张海伟[2] 郭变琴[1] 王云龙[1] 吴立翔[1] 

机构地区：[1]重庆市肿瘤研究所检验科,400030 [2]重庆市肿瘤研究所肿瘤学研究室,400030

出　　处：《检验医学与临床》2015年第6期729-731,共3页Laboratory Medicine and Clinic

基　　金：重庆市自然科学基金项目(cstc2013jcyjA1513)

摘　　要：目的探讨肝素锂抗凝血浆替代血清进行常规生化检测缩短正流转时间(TAT)的可行性。方法应用日立H7600-20全自动生化分析仪对抽取的110例患者肝素锂抗凝血浆和血清中的35项生化检测结果进行比对分析。结果在肝素锂抗凝血浆与血清的35项生化检测结果中,清蛋白(ALB)、γ-谷氨酰基转移酶(GGT)、总胆汁酸(TBA)、总胆红素(TB)、氯(Cl)、钙(Ca)、镁(Mg)、肌酐(CRE)、尿素(BUN)、尿酸(UA)、胱抑素C(CysC)、β2-微球蛋白(β2-MG)、肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)、乳酸脱氢酶(LDH)和高密度脂蛋白胆固醇(HDL-C)共16项指标的检测结果比较差异无统计学意义(P>0.05);而钠离子(Na+)、钾离子(K+)、二氧化碳(CO2)、磷(P)、铁(Fe)、丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、碱性磷酸酶(ALP)、总蛋白(TP)、前清蛋白(PreA)、α-L-岩藻糖苷酶(AFU)、三酰甘油(TG)、总胆固醇(TCH)、低密度脂蛋白胆固醇(LDL-C)、载脂蛋白A1(ApoA1)、载脂蛋白B(ApoB)、脂蛋白a[Lp(a)]、血糖(GLU)、直接胆红素(DB)共19项指标的检测结果比较差异有统计学意义(P<0.05)。结论肝素锂抗凝血浆与血清中常规生化检测结果不完全一致,肝素锂抗凝血浆可选择性地替代血清进行部分急诊生化检测以缩短TAT。Objective To investigate the feasibility of using heparin lithium anticoagulation plasma instead of serum for conducting the routine biochemical tests to shorten turn around time（TAT）.Methods 35 items of biochemical detection results in the anticoagulation plasma samples with lithium heparin and serum samples extracted from 110 patients were performed the comparative analysis by using the Hitachi H7600-20 automatic biochemical analyzer.Results Among the detection results of 35 items of biochemical tests in heparin lithium anticoagulation plasma and serum,there were no statistically significant differences in 16 items of ALB,GGT,TBA,TB,Cl,Ca,Mg,CRE,BUN,UA,CysC,β2-MG,CK,CK-MB,LDH and HDL-C between heparin lithium anticoagulation plasma and serum samples（P〉0.05）;while 19 items of Na^＋,K^＋,CO2,P,Fe,ALT,AST,ALP,TP,PreA,AFU,TG,TCH,LDL-C,ApoA1,ApoB,Lp（a）,GLU and DB had statistical differences between heparin lithium anticoagulation plasma and serum（P〈0.05）.Conclusion The routine biochemical detection results in heparin lithium anticoagulation plasma and serum samples are not entirely consistent.Heparin lithium anticoagulation plasma may selectively replace the serum sample for conducting partial emergency biochemical detection items to shorten TAT.

关 键 词：血浆 血清 常规生化检验 正流转时间 

分 类 号：R446.1[医药卫生—诊断学]