高效液相色谱法测定大气颗粒物PM2.5中16种多环芳烃  

Determination of 16 PAHs from atmospheric particulate matter PM 2.5 by HPLC

作　　者：曾涛[1] 胡国媛[1] 林玉娜[1] 刘莉治[1] 罗晓燕[1] 

机构地区：[1]广州市疾病预防控制中心,广东广州510440

出　　处：《中国卫生检验杂志》2015年第5期616-619,共4页Chinese Journal of Health Laboratory Technology

摘　　要：目的建立了一种高效液相色谱同时分离和测定大气颗粒物PM2.5中16种优控多环芳烃的方法。方法采用二氯甲烷超声提取,浓缩后经乙腈定容,以乙腈和水作为流动相,反相色谱法梯度淋洗分离后,由紫外检测器串联荧光检测器检测。结果 16种PAHs的分离效果较好,线性关系良好(r>0.999),平均回收率为77.5%~104.0%,相对标准偏差为1.1%~6.8%,LOD为0.1μg/L^2.3μg/L,LOQ为0.3μg/L^7.5μg/L,均符合方法学的要求。在采样点采集PM2.5样品,并进行定量分析。通过使用16种PAHs毒性当量因子,计算了各自的毒性当量。结论该方法方便、可靠、灵敏度高,可满足PM2.5中多环芳烃的日常检测要求。Objective To develop a method for simultaneous determination of 16 kinds of polycyclic aromatic hydrocarbons（ PAHs） in atmosphere particulate matter（ PM 2. 5） by high performance liquid chromatography（ HPLC）. Methods The samples were extracted by ultrasonic with dichloromethane,and brought to volume by acetonitrile after enrichment,separated by reversed phase HPLC and gradient elution with acetonitrile an water as mobile phase,and detected by ultraviolet-fluorescence detector. Results Separation and linear relationship（ r〉0. 999） of 16 kinds of PAHs were good. The average recovery ranged77. 5%-104. 0%,the relative standard deviation ranged 1. 1%-6. 8%,the LODs ranged 0. 1 μg / L-2. 3 μg / L,the LOQs ranged 0. 3 μg / L-7. 5 μg / L,which were in line of the methodology requirement. In the sampling site,the PM 2. 5 samples were collected for quantitative analysis. The toxic equivalent quantities were respectively calculated with 16 kinds of PAHs as toxic equivalent factors. Conclusion The method is simple,reliable,sensitive,and it can be used for determining the content of PAHs in atmospheric particulate matter.

关 键 词：液相色谱 PM2.5 多环芳烃 

分 类 号：O657.63[理学—分析化学]