RNA干扰SHBG基因表达对滋养细胞增殖的影响  

Effect of RNA silence SHBG gene on proliferation of trophoblasts

作　　者：范杰慧[1] 金镇[1] 孙倩[1] 息小雪[1] 

机构地区：[1]中国医科大学附属盛京医院,辽宁沈阳110004

出　　处：《中国优生与遗传杂志》2015年第2期3-4,7,共3页Chinese Journal of Birth Health & Heredity

基　　金：国家自然科学基金(81170591);沈阳市科学技术计划项目(F11-262-9-46)

摘　　要：目的探讨RNA干扰技术抑制SHBG基因表达对滋养细胞增殖的影响。方法在体外通过RNA干扰技术,将SHBG si RNA转染至原代培养的人绒毛滋养细胞中,MTT法检测滋养细胞增殖情况。结果成功将SHBG si RNA转染人绒毛滋养细胞;转染后24、48、72h,SHBG si RNA转染组的细胞生长速度显著减缓,正常对照组(未经转染的细胞)、空白对照组(只转染脂质体,无si RNA)和阴性对照组(转染非特异性si RNA)的组间比较差异均无统计学意义(P>0.05);转染组(SHBG si RNA-I,SHBG si RNA-II,SHBG si RNA-III)之间差异均无统计学意义(P>0.05),各转染组分别与各对照组的组间比较差异均有统计学意义(P<0.05)。结论 si RNA可以有效干扰人绒毛滋养细胞SHBG基因的表达,抑制滋养细胞增殖的增殖活性。Objective：To investigate the effect of silencing SHBG gene by RNA interference on proliferation of trophoblasts. Methods：Small interferening RNA（si RNA）specific-targeting SHBG gene was transfected into cultured human first trimester trophoblasts,cell proliferation was detected by MTT method. Results：SHBG si RNA was successfully transfected into human trophoblasts;In 24,48,72 h after transfection,the cell proliferation rate of transfection groups slowed down significantly;There was no significant difference between normal control group（trophoblasts without any transfection）,blank control group（transfect liposome,without si RNA）and negative control group（transfect nonspecific si RNA）（P0.05）;There was no significant difference between transfection groups（transfect SHBG si RNA-I,SHBG si RNA-II,SHBG si RNA-III respectively）（P0.05）;There was significant difference between transfection groups and control groups（P0.05）. Conclusion：Si RNA can interfere the expression of SHBG gene in trophoblasts effectively,which inhibits the proliferation of trophoblasts.

关 键 词：性激素结合球蛋白 滋养细胞 RNA干扰 细胞增殖 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]