妊娠期肝内胆汁淤积症患者外周血中维生素D水平及维生素D受体的表达相关性研究  

The correlation research about the concentrations of the vitamin D and the expression of the vitamin D receptor(VDR)in the peripheral blood of patients with intrahepatic cholestasis of pregnancy

作　　者：胡健蓉 徐健红[1] 黄小霞[1] 

机构地区：[1]广州市花都区妇幼保健院妇产科,广州510800

出　　处：《中国优生与遗传杂志》2015年第2期56-58,共3页Chinese Journal of Birth Health & Heredity

基　　金：广州市花都区医疗卫生科研项目(编号14-HDWS-059)

摘　　要：目的通过检测妊娠期肝内胆汁淤积症(ICP)患者外周血中维生素D的浓度及维生素D受体(VDR)的表达水平,来研究维生素D对ICP的影响。方法对35例轻度ICP、35例重度ICP及35例正常孕妇收集相关资料(孕周、妊娠并发症、维生素D摄入、钙片摄入等),分别采用酶联免疫吸附试验(ELISA法)及实时荧光定量检测三组孕产妇外周血中的维生素D含量及VDR m RNA的表达情况。结果 (1)重度ICP组外周血中维生素D的浓度及VDR m RNA的表达水平均明显低于轻度ICP组(t=5.21、15.512,P=0.000、0.000);(2)轻度ICP组外周血中维生素D的浓度与正常组比较无明显差异(t=1.06,P=0.23);(3)轻度ICP组外周血中VDR m RNA的表达水平明显低于正常组(t=-19.34,P=0.000)。结论重度ICP患者容易引起维生素D缺乏,VDR的表达在不同程度的ICP患者中均低于正常孕妇,ICP的发病可能与维生素D有一定的关系。Objective：To research the concentrations of the vitamin D and the expression of the vitamin D receptor（VDR）in the peripheral blood of patients with intrahepatic cholestasis of pregnancy（ICP）,so as to study the effects of vitamin D on ICP. Methods：To study 105 pregnant women,including 35 pregnant women with severe ICP、35 pregnant women with mild ICP and 35 normal pregnant women. A questionnaire about gestational weeks、pregnancy complication、vitamin D intake and calcium intake Was collected. Routine.Serum vitamin D levels were detected by enzyme linked immunosorbent assay（ELISA）.The expression of the VDR were genotyped by real time fluorescence polymerase chain reaction（RT-PCR）. Results：（1）Serum vitamin D levels and the expression of VDR at m RNA levels in the peripheral blood of severe ICP were significantly less than in the mild ICP（t=5.21、15.512,P=0.000、0.000）;（2）Serum vitamin D levels were not obvious different between in the mild ICP and the normal pregnant women;（3）The expression of VDR at m RNA levels in the peripheral blood of the mild ICP were significantly lower than in the normal pregnant women（t=-19.34,P=0.000）. Conclusions：Patients with severe ICP at high risk of vitamin D deficiency and the expression of VDR was low in the peripheral blood of patients with intrahepatic cholestasis of pregnancy,so the pathogenesis of ICP have a certain relationship with vitamin D.

关 键 词：维生素D 酶联免疫吸附试验 荧光定量 妊娠期肝内胆汁淤积症 

分 类 号：R714.25[医药卫生—妇产科学]