缬沙坦对糖尿病小鼠肾小球组织Notch通路活化及足细胞丢失的影响  

Effect of valsartan on Notch pathway activation and podocyte loss in glomeruli of diabetic mice

作　　者：高峰[1] 姚敏[2] 王晓梅[1] 刘淑霞[3] 段惠军[3] 

机构地区：[1]河北医科大学第三医院病理科,河北石家庄050051 [2]河北医科大学基础医学院生物化学研究室,河北石家庄050017 [3]河北医科大学基础医学院病理学教研室,河北石家庄050017

出　　处：《河北医科大学学报》2015年第1期1-5,共5页Journal of Hebei Medical University

基　　金：河北省自然科学基金(H2014206294);河北省医学科学研究重点课题(20130229)

摘　　要：目的探讨应用缬沙坦对糖尿病小鼠肾小球组织中Notch通路活化及足细胞丢失的影响。方法建立糖尿病小鼠模型给予缬沙坦治疗,观察小鼠肾小球细胞凋亡及足细胞脱落情况,采用Western blot检测Notch胞内域1(Notch intracellular domain 1,NICD1)、Hesl、Heyl、Bax、Bcl-2、cleaved caspase-3蛋白表达情况,Real-time PCR检测Hesl和Heyl mRNA表达情况。结果缬沙坦可降低糖尿病小鼠肾小球细胞凋亡及足细胞脱落,减少糖尿病小鼠肾小球组织中NICDl、Hesl、Heyl、Bax、cleaved caspase-3的表达,增加Bcl-2表达(P<0.05)。结论缬沙坦可通过抑制糖尿病小鼠肾小球组织中Notch通路的活化,减少足细胞凋亡和脱落。Objective To investigate the effect of valsartan on activation of Notch pathway and podocyte loss.Methods Apoptosis and podocyte detachment of glomeruli were measured after diabetic mice were treated with valsartan.The levels of Notch intracellular domain 1（NICD1）,Hesl,Heyl,Bax,Bcl-2 and cleaved caspase-3 were determined by Western blot.Realtime PCR was used to evaluate Hesl and Heyl mRNA expression.Results Valsartan suppressed cell apoptosis and podocyte detachment of glomeruli in diabetic mice.Valsartan also inhibited overexpression of NICD1,Hes1,Hey1,Bax and cleaved caspase-3,and increased Bcl-2 level in glomerular tissues of diabetic mice（P〈0.05）.Conclusion Valsartan inhibits the activation of Notch pathway in glomerular tissues of diabetic mice,also inhibits podocyte apoptosis and detachment.

关 键 词：糖尿病肾病 足细胞 肾小球 

分 类 号：R587.24[医药卫生—内分泌] R972.2[医药卫生—内科学]