高效液相色谱法同时测定降脂灵片中4种蒽醌类成分的含量  

Simultaneous determination of four anthraquinones in Jiangzhiling tablet by HPLC

作　　者：刘敏[1] 贾海红[1] 刘彦[2] 律涛[2] 樊淑彦[1] 

机构地区：[1]河北医科大学药学院药物分析学教研室,河北石家庄050017 [2]河北医科大学药学院实验中心,河北石家庄050017

出　　处：《河北医科大学学报》2015年第1期52-55,共4页Journal of Hebei Medical University

摘　　要：目的建立高效液相色谱法（high performance liquid chromatography,HPLC）同时测定降脂灵片中橙黄决明素、芦荟大黄素、大黄素和大黄素甲醚的含量。方法采用HPLC测定降脂灵片中橙黄决明素、芦荟大黄素、大黄素和大黄素甲醚的含量,色谱柱为Kromasil C_（18）柱（250 mm×4.6 mm,5μm）,以甲醇-0.1%磷酸为流动相梯度洗脱（0~5 min,40：60~70：30;5~10 min,70：30~80：20;10~15 min,80：20~90：10;15~25 min,90：10）,检测波长286 nm,流速1.0 mL/min,进样量20μL,柱温25℃。结果在选定的色谱条件下橙黄决明素、芦荟大黄素、大黄素、大黄素甲醚具有良好的分离度和稳定性。橙黄决明素在16.7~134 ng范围内线性关系良好,样品回收率为98.7%,相对标准偏差值（relative standard deviation,RSD）为0.3%;芦荟大黄素在11.1~89.1 ng范围内线性关系良好,样品回收率为98.5%,RSD为1.0%;大黄素在90.3~722 ng范围内线性关系良好,样品回收率为98.5%,RSD值为0.7%;大黄素甲醚在59.2~473 ng范围内线性关系良好,样品回收率为102.0%,RSD值为0.3%。结论HPLC同时测定降脂灵片中橙黄决明素、芦荟大黄素、大黄素和大黄素甲醚的含量,快速、简便、准确、专属性高,可以用于降脂灵片的质量控制。Objective To develop a simultaneous analysis method for Aurantio-obtusifolin,Aloeemodin,Emodin and Physcion in J iangzhiliing tablet by HPLC.Methods Determination of the Aurantio-obtusifolin,Aloeemodin,Emodin and Physcion in J iangzhiliing tablet by HPLC,the four constituents were separated on an Kromasil C_（18） column（250 mm×4.6 mm,5 μm） using a mobile phase consisted of methanol —0.1%phosphoric acid in water at the detection wavelength of 286 nm,gradient elution program（0 — 5 min,40：60 — 70：30;5— 10 min,70：30-80：20;10— 15 min,80：20—90：10;15—25 min,90：10）,the flow rate was 1.0 mL/min,the injection volume 20 μL and the column temperature 25 ℃.Results Aurantio-obtusifolin,Aloeemodin,Emodin and Physcion were separated from imputities well.Aurantio-obtusifolin showed a good linear relationship in the range of 16.7—134 ng,the average recovery was 98.7%,and its RSD rate 0.3%;the range of Aloeemodin 11.1—89.1 ng,the average recovery 98.5%,and its RSD rate 1.0%;the range of emodin 90.3—722 ng,the average recovery 98.5%,and its RSD rate 0.7%;Physcion in range of 59.2—473 ng,the average recovery 102.0%,and its RSD rate0.3%.Conclusion The method is rapid,simple,accurate and with good reproducibility and can be used in quality control of Jiangzhiling tablet.

关 键 词：降血脂药物 蒽醌类 色谱法 高压液相 

分 类 号：R972.6[医药卫生—药品]