结核感染T细胞检测在骨关节结核中的诊断价值  

Application value of tuberculous infection of T cells spot test in diagnosing osteoarticular tuberculosis

作　　者：贾晨光[1] 姚黎明[1] 李秀武[2] 刘丰胜[1] 章志华[3] 张金文[3] 

机构地区：[1]河北省胸科医院骨科,河北石家庄050041 [2]河北省胸科医院病理科,河北石家庄050041 [3]河北省胸科医院科教处,河北石家庄050041

出　　处：《河北医科大学学报》2015年第2期148-151,共4页Journal of Hebei Medical University

基　　金：河北省科学技术研究与发展计划项目(12277754)

摘　　要：目的评价结核感染T细胞检测(tuberculous infection of T cells spot test,T-SPOT.TB)在疑似骨关节结核病中的诊断价值。方法选取80例确诊为骨关节结核患者和44例排除结核患者。分别进行抗酸染色、结核菌培养、实时荧光定量PCR检测及T-SPOT.TB,比较并分析检测结果。结果 T-SPOT.TB的敏感度、特异度和准确度分别为82.5%、75.0%、79.8%。T-SPOT.TB的阳性检出率显著高于抗酸染色、结核分枝杆菌培养(P<0.05),而在不同疾病部位(脊柱、四肢关节)及不同病程分组间阳性检出率差异无统计学意义(P>0.05)。结论 TSPOT.TB可以作为骨关节结核病的重要辅助诊断方法。Objective To investigate the value of tuberculous infection of T cells spot test（T-SPOT.TB） in the diagnosis of osteoarticular tuberculosis.Methods The study enrolled 80 patients who were confirmed osteoarticular tuberculosis and 44 did not have active tuberculosis.Acid-fast staining,mycobacterial culture,real-time quantitative PCR were respectively performed for the patients.The results were compared and analyzed.Results The sensitivity,specificity,accuracy for osteoarticular tuberculosis diagnosis by T-SPOT.TB assay were 82.5%,75.0%and79.8%.The positive rate of T-SPOT.TB was significantly higher than that of acid-fast staining,mycobacterial culture（P〈0.05）.But,there were no statistical difference in different locations（spine or extremities joint） and courses between groups.Conclusion The results suggested that T-SPOT.TB assay could provide useful aids in the diagnosis of osteoarticular tuberculosis.

关 键 词：结核 骨关节 结核感染T细胞检测 诊断 

分 类 号：R529.2[医药卫生—内科学]