甩片改良抗酸法与FQ-PCR技术在脑脊液抗酸菌检查中的应用分析  

Analysis of acid fast bacilli in cerebrospinal fluid with modified acid-fast method and FQ-PCR technique

作　　者：吕翠环[1] 李玉静[1] 孙志平[1] 李卓[2] 吕莎[3] 郑立恒[1] 

机构地区：[1]河北省胸科医院检验科,河北石家庄050041 [2]河北省胸科医院骨科,河北石家庄050041 [3]河北省胸科医院呼吸科,河北石家庄050041

出　　处：《河北医科大学学报》2015年第2期170-172,共3页Journal of Hebei Medical University

基　　金：河北省科学技术研究与发展计划项目(13277794D);河北省科学技术研究与发展指导计划项目(132777269)

摘　　要：目的评价脑脊液改良抗酸染色法与实时荧光定量聚合酶链反应(fluorescence quantitative polymerase chain reaction,FQ-PCR)法诊断结核性脑膜炎(tuberculous meningitis,TBM)的意义。方法 TBM患者81例,采集脑脊液检测标本,分别采用离心甩片改良抗酸染色法、实时FQ-PCR法检测抗酸杆菌。结果 81例改良抗酸染色法阳性率66.7%(54/81)高于FQ-PCR方法阳性率50.6%(41/81),差异有统计学意义(P<0.05)。结论在诊断TBM方面,改良抗酸染色方法显著优于FQ-PCR方法。Objective To evaluate united test significance in the diagnosis of tuberculous meningitis（TBM） with modified acid-fast staining method of cerebrospinal fluid and real-time fluorescence quantitative polymerase chain reaction（FQ-PCR）.Methods Cerebrospinal fluid samples from 81 patients with TBM were detected for Mycobacterium tuberculosis respectively with centrifugal modified acid-fast staining method and real-time FQ-PCR.Results The positive rate of modified acid-fast staining 66.7%（54/81） was significantly higher than that of FQ-PCR50.6%（41/81）,there was significant difference between the two methods（P〈0.05）.Conclusion Modified acid-fast staining method is significantly better than FQ-PCR in the diagnosis of TBM.

关 键 词：结核 脑膜 染色与标记 实时聚合酶链反应 

分 类 号：R529.3[医药卫生—内科学]