Current status of genetic transformation technology developed in cucumber(Cucumis sativus L.)  

作　　者：WANG Shun-li Seong Sub Ku YE Xing-guo HE Cong-fen Suk Yoon Kwon Pil Son Choi 

机构地区：[1]Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture/Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences [2]Department of Oriental Pharmaceutical Development, Nambu University [3]Institute of Crop Science, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement [4]School of Sciences, Beijing Technology and Business University [5]Plant Systems Engineering Research Center

出　　处：《Journal of Integrative Agriculture》2015年第3期469-482,共14页农业科学学报（英文版）

基　　金：financially supported by grants from the Biogreen 21 Program, RDA, Korea (PJ00810304);the Agricultural Science and Technology Innovation Program (ASTIP) of Chinese Academy of Agricultural Sciences (2014–2015);the Beijing Municipal Education Commission, China (KM200910011001)

摘　　要：Genetic transformation is an important technique for functional genomics study and genetic improvement of plants. Until now, Agrobacterium-mediated transformation methods using cotyledon as explants has been the major approach for cucumber, and its frequency has been up to 23%. For example, significantly enhancement of the transformation efficiency of this plant species was achieved from the cotyledon explants of the cultivar Poinsett 76 infected by Agrobacterium strains EHA105 with efficient positive selection system in lots of experiments. This review is to summarize some key factors influencing cucumber regeneration and genetic transformation, including target genes, selection systems and the ways of transgene introduction, and then to put forward some strategies for the increasing of cucumber transformation efficiency. In the future, it is high possible for cucumber to be potential bioreactor to produce vaccine and biomaterials for human beings.Genetic transformation is an important technique for functional genomics study and genetic improvement of plants. Until now, Agrobacterium-mediated transformation methods using cotyledon as explants has been the major approach for cucumber, and its frequency has been up to 23%. For example, significantly enhancement of the transformation efficiency of this plant species was achieved from the cotyledon explants of the cultivar Poinsett 76 infected by Agrobacterium strains EHA105 with efficient positive selection system in lots of experiments. This review is to summarize some key factors influencing cucumber regeneration and genetic transformation, including target genes, selection systems and the ways of transgene introduction, and then to put forward some strategies for the increasing of cucumber transformation efficiency. In the future, it is high possible for cucumber to be potential bioreactor to produce vaccine and biomaterials for human beings.

关 键 词：Cucumis sativus L. plant regeneration genetic transformation positive selection system 

分 类 号：S642.2[农业科学—蔬菜学]