N-乙酰半胱氨酸对快速上浮脱险致减压病大鼠心肺组织的影响  

Effect of N-acetylcysteine on lung and heart injury of rats with a fast floating escape induced decompression sickness

作　　者：王芳芳[1] 方以群[1] 攸璞[1] 包晓辰[1] 马骏[1] 张师[1] 

机构地区：[1]海军医学研究所,上海200433

出　　处：《军事医学》2015年第2期89-91,共3页Military Medical Sciences

基　　金：海军后勤部科研基金资助项目(12A106);全军医学科研"十二五"重点项目(BWS11J053;BHJ12J003)

摘　　要：目的研究N-乙酰半胱氨酸(NAC)对快速上浮脱险致减压病大鼠心肺组织的影响。方法雄性SD大鼠80只,随机分为4组,每组20只。实验组于进舱前1 h通过腹腔注射NAC溶液(注射剂量分别为250、500和1000 mg/kg),对照组于进舱前1 h腹腔注射相同体积生理盐水。压缩空气以指数速率2t/7加压至1.5 MPa,停留4min后匀速减压至常压出舱。出舱后即观察大鼠行为学表现并统计存活率,出舱后0.5 h活杀大鼠,取心、肺组织,观察其病理变化。结果 NAC 500 mg/kg组大鼠存活率(90%)显著高于对照组(65%)(P<0.05);对照组大鼠肺泡结构大面积破坏融合,肺泡腔内可见红细胞渗出;心肌纤维水肿、变性,断裂明显。而NAC组大鼠肺泡壁增厚程度和心肌纤维水肿程度均较对照组轻微。结论 NAC可通过减轻大鼠心肺组织的损伤和炎症预防快速上浮脱险所致减压病的发生。Objective To investigate the effect of N-acetylcysteine（ NAC） on lung and heart injury of rats with a fast floating escape induced decompression sickness. Methods Eighty male Sprague-Dawley rats were randomly and evenly divided into four groups： control group and three NAC prevention groups. The NAC groups were treated with different doses of NAC（ 250,500 or 1000 mg / kg） by intraperitoneal injection 1 h before entrance. In the control group,rats were given an equal volume of saline1 h before entrance. The air was pressurized at the 2t /7exponential rate to 1. 5 MPa which was maintained for 4 min and then uniformly decompressed to atmospheric pressure. The extravehicular survival and pathological changes in the lung and heart tissue were detected 0. 5 h after rat egress. Results The survival rate of rats treated with NAC 500 mg / kg（ 90%） was significantly higher than that of those treated with saline（ 65%） alone（ P 0. 05）. There was large break and fusion in the structure of pulmonary alveolus of control group besides obvious erythrocyte exudation,cardiac muscle fibers edema,and obvious denaturation and break. Conclusion NAC can play a protective role in rats with a fast floating escape induced decompression sickness by mitigating the injury to and inflammation of lung and heart tissue.

关 键 词：N-乙酰半胱氨酸 快速上浮脱险 减压病 心脏 肺脏 

分 类 号：R852.16[医药卫生—航空、航天与航海医学]