慢性乙型病毒性肝炎患者外周血辅助性T细胞9及白细胞介素9的表达及临床意义  

Expression and clinical significance of T helper 9 cells and interleukin-9 in chronic hepatitis B patients

作　　者：孙丽[1] 李红胜[1] 阮玲娟[1] 任振唤[2] 寇筱囡[3] 

机构地区：[1]浙江省嘉兴市第二医院检验科,浙江嘉兴314000 [2]浙江省丽水市人民医院检验科,浙江丽水323000 [3]黑龙江省大庆油田总医院检验科,黑龙江大庆163316

出　　处：《疾病监测》2015年第1期35-37,共3页Disease Surveillance

摘　　要：目的观察慢性乙型病毒性肝炎(乙肝,CHB)患者外周血中辅助性T淋巴细胞9(Th9)、白细胞介素9(IL-9)水平变化,并探讨其临床意义。方法选择2012年1月至2013年4月在嘉兴市第二医院就诊的慢性乙肝患者53例作为实验组,同时选择健康体检者32例为对照组。分别检测血清丙氨酸转氨酶(ALT);外周血Th9细胞;血清中细胞因子IL-9的水平。比较两组数据并分析ALT与各指标关系。结果 CHB患者外周血中Th9比例显著高于健康对照组[分别为(1.02±0.62)%、(0.33±0.10)%,P<0.05],IL-9水平高于健康对照组[分别为(184.63±50.32)pg/ml、(82.71±10.55)pg/ml,P<0.05],ALT轻度异常组、中度异常组、重度异常组Th9细胞比例分别为[(0.42±0.17)%,(1.14±0.33)%,(1.51±0.89)%],3组间差异有统计学意义(均P<0.05);IL-9水平分别为[(104.76±12.42)pg/ml,(162.51±47.79)pg/ml,(297.14±81.56)pg/ml],3组间差异有统计学意义(均P<0.05)。CHB患者外周血Th9的表达与ALT(r=0.637,P<0.05)和IL-9的水平(r=0.817,P<0.01)均呈正相关。血清IL-9的水平与ALT也呈正相关(r=0.576,P<0.01)。结论 CHB患者外周血Th9细胞比例及l L-9水平显著升高,且与炎症指标ALT明显相关,提示Th9及IL-9参与CHB的发病及病情发展。Objective To detect the expression of T helper cells （Th9） and the level of interleukin-9 （IL-9） in the peripheral blood of patients with chronic hepatitis B. Methods Fifty three chronic hepatitis B patients seeking medical care in our hospital from January 2012 to April 2013 were included in the study. And 32 people receiving health examination were used as controls. ALT level and the expression of The cells and IL-9 level were detected. The data of two groups were compared and analyzed. Results The expression of The cells and the level of IL-9 of chronic hepatitis B patients[ （ 1.02 ± 0. 62 ） %, （ 184. 63 ± 50. 32 ） pg/ml ] were significantly higher than those of controls E （ 0. 33 ± 0. 10）% , （297.14 ±81.56） pg/ml] , respectively （all P 〈 0.05 ）. The expression of Th9 cells in groups with mild, moderate, or significant elevated ALT were （ 0.42 ± 0. 17 ） % , （ 1.14 ± 0. 33 ） % and （ 1.51 ± 0. 89 ） % , respectively, the differences had statistical significance （P 〈 0.05 ）. The levels of IL-9 in groups with mild, moderate, or significant elevated ALT were （ 104. 76 ± 12.42） pg/ml, （ 162. 51 ±47.79） pg/ml and （297.14 ± 81.56） pg/ml, respectively, the differences had statistical significance （ P 〈 0. 05 ）. The expression of Th9 cells was positively correlated with ALT level （ r = 0. 637, P 〈 0. 05 ） and IL-9 level （ r = 0. 817, P 〈 0. 01 ）. The level of IL-9 was positively correlated with ALT level （ r = 0. 576,P 〈 0.01 ）. Conclusion The expression of The cells and IL-9 level in the chronic hepatitis B patients were obviously high, and correlated with inflammatory indicator ALT level. These results indicate that The cells might participate in the pathogenesis of chronic hepatitis B.

关 键 词：慢性乙型病毒性肝炎 T淋巴细胞 辅助诱导 白细胞介素-9 丙氨酸转氨酶 

分 类 号：R512.62[医药卫生—内科学]