加味凉膈散对脂多糖刺激小鼠血小板活化标志物及炎症因子释放的影响  

The impact of modified Liangge powder on platelet activation markers and release of proinflammatory cytokine of mice by stimulation of lipopolysaccharide

作　　者：张静姝[1] 王勇强[2] 王兵[2] 刘学政[3] 

机构地区：[1]天津中医药大学,天津300193 [2]天津市第一中心医院,天津300192 [3]天津中医药大学第一附属医院,天津300193

出　　处：《中国中西医结合急救杂志》2015年第2期133-137,共5页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

基　　金：国家临床重点专科建设项目（2011873）;天津市中医药管理局中医中西医结合科研专项基金（11085）

摘　　要：目的：观察加味凉膈散对脂多糖（LPS）刺激小鼠血小板活化标志物及炎症因子释放的影响。方法将112只雄性昆明小鼠按随机数字法分为对照组、模型组以及加味凉膈散低、中、高剂量组。采用小鼠尾静脉注射LPS 10 mg/kg的方法制备脓毒症模型，对照组小鼠尾静脉注射等量生理盐水。加味凉膈散组分别以低、中、高剂量0.94、1.89、2.84 g/mL加味凉膈散0.02 mL/g灌胃3 d，对照组和模型组给予等量生理盐水灌胃3 d。不同剂量加味凉膈散组及模型组于制模后24 h、72 h各处死8只小鼠取血，对照组于制模型后24 h处死8只小鼠取血进行检测；用血细胞分析仪检测血浆血小板计数（PLT）；用酶联免疫吸附试验（ELISA）检测白细胞介素-10（IL-10）、高迁移率族蛋白B1（HMGB1）、血小板第4因子（PF4）水平。各组于制模后72 h另取8只小鼠用激光扫描共聚焦显微镜检测Ca^2＋浓度。结果与对照组比较，制模后24 h模型组PLT（×10^9/L：347.70±115.10比1013.10±136.60）水平即降低，IL-10（μg/L：356.86±34.72比39.50±23.45）、HMGB1（mg/L：16.24±4.49比10.75±1.91）、PF4（μg/L：5.43±0.61比1.33±0.40）、Ca^2＋（nmoL/L：8.60±0.52比1.05±0.33）水平即升高，持续到制模后72 h。与模型组比较，加味凉膈散低、中、高剂量组PLT水平均明显升高，以中剂量组制模后72 h升高更显著（×10^9/L：952.13±104.02比771.50±129.30，P＜0.05）；加味凉膈散低、中、高剂量组IL-10、HMGB1、PF4、Ca^2＋水平均明显降低，IL-10以高剂量组制模后72 h（μg/L：110.17±29.12比441.50±30.72）、HMGB1以高剂量组制模后24 h（mg/L：10.33±3.52比16.24±4.49）、PF4以中剂量组制模后24 h（μg/L：2.08±0.92比5.43±0.61）、Ca^2＋以高剂量组（nmoL/L：2.97±0.96比8.60±0.52）降低更显著（均P＜0.05）。结论加味凉膈散可能通过下调LPS诱发的炎症细胞因子释放、抑制血小板Ca^2＋的�Objective To observe the impact of modified Liangge powder （MLP） on platelet activation markers and the release of proinflammatory cytokine in mice by stimulation of lipopolysaccharide （LPS）. Methods 112 male mice were randomly divided into control group, model group and MLP low, middle and high dose treatment groups. The sepsis model was reproduced by injection of LPS 10 mg/kg into a mouse tail vein. In the control group, normal saline 10 mg/kg was injected into the tail vein of mouse. The MLP low, middle, and high dose groups received 0.94, 1.89, 2.84 g/mL MLP 0.02 mL/g by gavage respectively for 3 days, while the control group and model group received equal amount of normal saline by gavage for 3 days. After modeling for 24 hours and 72 hours, 8 mice in each of the three different dose MLP groups and model group were killed and their blood was taken. In the control group, after modeling for 24 hours, 8 mice were killed and their blood was taken. Platelet （PLT） was counted by blood cell analyzer, plasma interleukin-10 （IL-10）, high mobility group protein B1 （HMGB1） and platelet factor 4 （PF4） were detected by enzyme-linked immunosorbent assay （ELISA）. In each group after modeling for 72 hours, another 8 mice were taken, and laser scanning confocal microscopy was used to measure the platelet cytosolic Ca^2＋ concentration. Results Compared with the control group, the level of PLT at 24 hours（×10^9/L： 347.70±115.10 vs. 1 013.10±136.60） was decreased, and the levels of IL-10 （μg/L： 356.86±34.72 vs. 39.50±23.45）, HMGB1 （mg/L： 16.24±4.49 vs. 10.75±1.91）, PF4 （μg/L： 5.43±0.61 vs. 1.33±0.40） and Ca^2＋ （nmoL/L： 8.60±0.52 vs. 1.05±0.33） were elevated in model group. Compared with the model group, the levels of PLT in the MLP high, middle and low dose groups were all significantly elevated; the increase in PLT in middle dose group after modeling for 72 hours was the most remarkable （×10^9/L：952.13±104.02 vs. 771.50±129.30, P 〈 0.05）; the

关 键 词：脂多糖 脓毒症 加味凉膈散 血小板活化标志物 炎症因子 

分 类 号：R554.6[医药卫生—血液循环系统疾病]