四川小麦品种(系)中慢锈基因Lr 34/Yr 18/Pm 38的分子检测  

Molecular Characterization of Slow-Rusting Genes Lr 34/Yr 18/Pm 38 in Sichuan Wheat Varieties(Lines)

作　　者：刘光辉[1] 杨随庄[1,2] 黄可兵[1,2] 杨仕雷[1,2] 

机构地区：[1]西南科技大学生命科学与工程学院,四川绵阳621010 [2]西南科技大学小麦研究所,四川绵阳621010

出　　处：《种子》2015年第3期1-5,共5页Seed

基　　金：西南科技大学科研基金项目(编号:11 zx 7113);四川省攻关项目(编号:2011 NZ 0098-3);四川省教育厅项目(编号:12 zd 1102)

摘　　要：慢锈性基因Lr 34/Yr 18/Pm 38对小麦叶锈病和条锈病都具有广谱持久抗性,为明确该基因在四川小麦品种（系）中的分布,对90份四川小麦品种（系）进行慢锈病基因Lr 34/Yr 18/Pm 38的分子检测。利用与Lr 34/Yr 18/Pm 38基因位点紧密连锁的STS标记cs LV 34和基于该基因第11外显子（exon 11）等位变异开发的4对功能标记cssfr 1、cssfr 2、cssfr 3、cssfr 4检测90份四川小麦品种（系）。结果表明,所检测的90份四川小麦材料中都不含Lr 34/Yr 18/Pm 38位点,功能标记cssfr 1~cssfr 4检测结果与STS标记cs LV 34检测结果一致。结论：四川小麦品种（系）含有Lr 34/Yr 18/Pm 38基因位点的材料较少,在过去育种中,Lr 34/Yr 18/Pm 38基因不被人们重视,没有得到很好的利用。The slow-rusting gene Lr 34/Yr 18/Pm 38 was conferred broad spectrum resistances to leaf rust and stripe rust. The aim of this study was to verify the distribution of the slow-rusting gene Lr 34/Yr 18/Pm 38 in90 Sichuan wheat varieties（lines）. A total of 90 sichuan wheat varieties were tested using a STS marker cs LV 34 and 4 functional markers cssfr 1- cssfr 4 which were developed based on a 3-bp deletion in the exon 11 of Lr 34/Yr 18/Pm 38 gene. The result indicated that none of lines were characterized to have Lr 34/Yr 18/Pm38 resistance gene. The results tested by the STS marker cs LV 34 showed no difference to those of cssfr 1- cssfr4. The fact that the ratio of Sichuan wheat varieties carrying Lr 34/Yr 18/Pm 38 was very low,implied that this slow-rusting gene don＇t attach much weight in the core collection.

关 键 词：四川省 小麦品种(系) LR 34/Yr 18/Pm 38 条锈病 分子检测 

分 类 号：S512.1[农业科学—作物学]