IBDV感染不同阶段雏鸡睾丸NGF表达  

Expression Change of Nerve Growth Factor in Testis of Chicken Infected with IBDV

作　　者：陈玲丽[1] 阮涛[1] 李冲[1] 孙相和 葛亚明[1] 刘明成[1] 姚建龙 王欢[1] 宁红梅[1] 

机构地区：[1]河南科技学院动物科学学院,河南新乡453003 [2]伊宁县动物卫生监督所,新疆伊宁835100

出　　处：《西北农业学报》2015年第3期44-47,共4页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：河南省教育厅基金(12B230003);国家级大学生创新创业训练计划项目(201210467011)

摘　　要：研究鸡传染性法氏囊病病毒(Infection bursal disease virus,IBDV)不同感染阶段对鸡睾丸中神经生长因子(Nerve growth factor,NGF)表达量的影响。通过点眼、滴鼻和擦肛途径对3周龄雏鸡人工接种IBDV后,分别采集不同感染阶段的雏鸡睾丸,利用半定量PCR和免疫组织化学检测睾丸中NGF表达量的变化。结果表明,IBDV感染后48h和72hNGF表达量显著低于对照组(P<0.05),其他阶段差异不显著。推测NGF表达水平可能与睾丸的损伤有关,雄激素的分泌在IBD的病程中发挥着重要作用。The purpose of this study is to explore the expression of the nerve growth factor（NGF）in testis of chickens at different stages of IBDV infection.3weeks of chickens were vaccinated with IBDV through eye,nasal and cloaca,and the testis were collected at different infection stages to determine the expression of NGF by semi-quantitative PCR and immunohistochemistry.The results showed that the target fragments,length of 219 bp,277bp and 414 bp were uniform with theories by detecting NGF andβ-actin at different stages and IBDV after 72 hinfection by agarose electrophoresis gel.The expression of NGF of testis in the experimental group were significantly increased at 48hpost-infection compared with control group.The expression levels of NGF were changed with the damage extents of the testis post-infection with IBD.The results suggested that the levels of androgen might play an important role in process of IBDV.

关 键 词：IBDV感染 雏鸡 睾丸 神经生长因子(NGF) 

分 类 号：S858.31[农业科学—临床兽医学]