甜荞花同源异型基因FeMADS1的克隆和序列结构分析  

Cloning and Sequence Analysis of FeMADS1 Gene from Fagopyrum esculentum

作　　者：方正武[1] 刘志雄[1,2] 

机构地区：[1]长江大学农学院,作物遗传育种研究所,湖北荆州434025 [2]长江大学园艺园林学院,湖北荆州434025

出　　处：《西北农业学报》2015年第3期82-87,共6页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家自然科学基金(31101202);国家公益行业(农业)科研专项(201303008)

摘　　要：采用同源克隆方法,并结合RACE技术,从甜荞花芽分离得到1个A类MADS-box基因FeMADS1的cDNA全长,GenBank登录号为KM386627,其cDNA全长1 107bp,包括1个编码234个氨基酸、长为705bp的开放阅读框。序列同源比对和分子系统发生分析表明,其蛋白与拟南芥AGL8(FUL)的相似性最高,属A类MADS-box基因亚家族中的euFUL进化系,含MADS、I、K和C末端4个明显的结构域,并且K结构域包含K1、K2和K3共3个保守的富含疏水氨基酸残基的亚结构域,C末端结构域含FUL型基因2个特有的模体:FUL motif和paleoAP1motfi。The cDNAs of an A-class MADS-box gene,FeMADS1,was cloned by homologous cloning and RACE method based on the sequence database of NCBI.The full length of FeMADS1 cDNA was 1 107 bp,which included an open reading frame（ORF）of 705 bp and coding for a polypeptide of234 amino acid residues.Conceptual translation revealed that FeMADS1 protein had four domains：MADS,I,K and C-terminal domain.The C terminal region harbored two highly conserved FUL motif and paleoAP1 motif.Three highly conserved regions contained expected hydrophobic amino acids in the K-domain,namely subdomains K1,K2 and K3.

关 键 词：甜荞 MADS-BOX基因 FeMADS1 花发育 

分 类 号：Q943.2[生物学—植物学]