哈尔滨市男男同性恋HIV-1感染者gag区基因分析  

Analysis on the characteristics of HIV-1 gag gene among men who have sex with men living with HIV-1 in Harbin City

作　　者：孙冬莹[1] 邵冰 张春蕾 李春兰[4] 杜娟[5] 宋波[4] 林元龙[4] 李庆海[6] 岳超[6] 金刚[6] 刘树林[6] 王滨有[1] 王福祥[4] 

机构地区：[1]哈尔滨医科大学公共卫生学院流行病教研室,哈尔滨市150086 [2]吉林医药学院公共卫生学院流行病教研室 [3]哈尔滨市胸科医院检验科 [4]哈尔滨医科大学附属第四医院感染科 [5]吉林医药学院药学院基础化学实验室 [6]哈尔滨医科大学基因组中心,哈尔滨市150086

出　　处：《中华实验和临床感染病杂志（电子版）》2015年第1期14-17,共4页Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition)

基　　金：黑龙江省自然科学基金(No.H201383)

摘　　要：目的了解哈尔滨市男男同性恋者(MSM)感染HIV-1流行株的亚型分布状况及基因变异情况。方法采集MSM人群中HIV感染者血液标本,提取病毒RNA并应用巢式聚合酶链反应(nested-PCR)对HIV病毒gag区基因进行扩增,并对扩增产物测序。Mega 5.1软件进行系统进化树分析及基因离散距离的计算,Bio Edit 7.0.0软件将相应的基因序列翻译成氨基酸并分析其变异情况。结果成功获得61条gag基因序列,亚型分析结果显示,CRF01_AE 49例(80.32%),CRF07-BC 5例(8.19%),B亚型7例(11.47%)。其中B亚型基因离散距离最大(0.078±0.005),CRF01_AE最小(0.051±0.004)。CRF01_AE在MSM人群中形成两个独立的进化簇。各亚型的MHR序列变异对比结果显示,B亚型的中MHR序列中第7位缬氨酸(V)突变为异亮氨酸(I)的比例最大。结论哈尔滨市MSM人群中主要存在CRF01-AE、CRF07-BC和B三种亚型,以CRF01-AE为主要流行亚型;其中B亚型基因变异程度最大。对MSM人群HIV-1流行及变异情况的监测有助于对MSM人群开展及时有效的治疗。Objective To determine the distribution of HIV-1 subtype and the genetic variation among men who have sex with men （MSM） infected with HIV-1 in Harbin. Methods Blood samples were collected from MSM infected with HIV, and viral RNA was extracted, HIV-1 gag gene was amplified by nested-PCR, and the subsequent products were carried out for sequencing. The phylogenetic analysis and calculated gene discrete distance were done by Mega 5.1 software. The variation of amino acid sequences were analyzed by BioEdit 7.0.0 software. Results According to the 61 gag gene sequences obtained, subtype analysis showed. Among them 49 samples （80.32%） were CRF01-AE, 5（80.32%） samples were CRF07-BC （8.19%）, 7 （11.47%） samples were B subtype. In which gene discrete distance of B subtype was maximum （0.078 -4- 0.005）, CRF01_AE was minimum （0.051 ：t： 0.004）. The result of analysis the variation of major homology region （MHR） sequence of gag gene region showed that the seventh valine （V） was mutated to isoleucine （I） owned the largest proportion in subtypes B comparison with the others. Conclusions The CRF01-AE, CRF07-BC and B subtypes of HIV-1 co-circulation among HIV infected MSM in Harbin. CRF01-AE was the main epidemic strain ofHIVol. Where subtype B owned the maximum of genetic variability.Monitoring the variation of HIV-1 epidemic among MSM could contribute to treatment for MSM timely and effectively.

关 键 词：男男同性恋 GAG基因 HIV-1亚型 

分 类 号：R512.91[医药卫生—内科学]