中朝边境地区鼠中斑点热立克次体的调查研究  

Epidemiological survey of spotted fever group rickettsia in rodents in northeastern China

作　　者：王卓[1] 于淼[1] 杨慎江 邱广斌 翟如波 郭醉元[1] 冯立[1] 吴益民[1] 

机构地区：[1]沈阳军区疾病预防控制中心 [2]解放军65835部队卫生队 [3]解放军第二O二医院检验科

出　　处：《中国预防医学杂志》2015年第3期184-186,共3页Chinese Preventive Medicine

基　　金：国家科技重大专项(2013ZX10004-203);辽宁省自然科学基金课题(20092204)

摘　　要：目的了解中朝边境地区鼠类感染斑点热群立克次体（SFGR）情况。方法运用聚合酶链式反应（PCR）和间接免疫荧光（IFA）对吉林、辽宁采集的野鼠样本进行ompA基因片段检测和血清抗体调查。结果 PCR检测鼠脾样本共132份,阳性14份,阳性率10.61%。其中,集安和宽甸阳性率分别为7.58%、13.64%,二者差异无统计学意义（P=0.3964）。不同鼠种检测阳性率以黑线姬鼠为高13.79%,次为林姬鼠8.0%。IFA检测鼠血清115份,阳性20份,阳性率17.39%。其中集安、宽甸、珲春阳性率阳性率分别为11.76%、14.63%、25%,三地鼠抗体阳性率差异无统计学意义（P=0.2755）。不同鼠种中,除褐家鼠和鼩鼱外,其余鼠种检出阳性率均较高（16.88%~33.33%）。结论调查地区野鼠中SFGR感染普遍,说明存在SFGR自然疫源地。Objective To understand the epidemiological features of spotted fever group rickettsia（SFGR）in rodents in northeastern China. Methods Spleen and blood samples from wild rodents captured in Jilin and Liaoning were collected.ompA gene of SFGR was amplified by PCR and serum antibody was tested by IFA.Results Out of 132 rodent spleen samples,14 were PCR positive with the positive rate of 10.61%（14/132）.The positive rates in Jinan and Kuandian were 7.58% and 13.64% with no significant difference（P=0.3964）.The highest positive rate of 13.79% was found in Apodemus agrarius.Out of 115 serum samples,20 were found positive for antibody with the positive rate of 17.39%.The antibody positive rates were11.76%,14.63% and 25%in Jian,Kuandian,and Huichun with no statistical difference（P=0.2755）.Conclusions SFGR infection is very common in wild rodents in northeastern region of China,indicating that this area is a natural focus of SFGR in China.

关 键 词：斑点热群立克次体 宿主动物 PCR 间接免疫荧光 

分 类 号：R376.3[医药卫生—病原生物学]