Bmi-1基因对人类胚胎纹状体来源神经干细胞凋亡的影响  

Effect of Bmi-1 on apoptosis of human fetal striatum derived neural stem cells

作　　者：赵春松[1,2] 邹海强[3] 闫晓明[4] 陈凌[5] 王佳茵[1,2] 关云谦[1,2] 张愚[1,2] 

机构地区：[1]首都医科大学宣武医院细胞生物室,北京100053 [2]教育部神经变性病重点实验室,北京100053 [3]广州军区广州总医院神经内科,广州510010 [4]首都医科大学宣武医院功能神经外科,北京100053 [5]首都医科大学宣武医院神经外科,北京100053

出　　处：《首都医科大学学报》2015年第1期103-110,共8页Journal of Capital Medical University

基　　金：国家重点基础研究发展计划资助项目(973计划)(2012CBA01300);国家高技术研究发展计划资助项目(863计划)(2011AA020106);北京市自然科学基金(7102078);北京市科技新星项目(2009B22);北京市科委健康培育项目(Z111107067311033);北京市教育委员会科技计划重点项目(KZ201310025024)~~

摘　　要：目的探讨Bmi-1基因对人类胚胎纹状体来源神经干细胞（neural stem cell,NSC）凋亡的影响。方法提取16~18周自然流产胚胎脑组织纹状体的NSC,体外培养至3~5代,用慢病毒载体对其进行Bmi-1基因的RNA干扰和过表达回复。观察Bmi-1基因对体外NSC凋亡的影响。结果在感染后2 h和3 d两个时间点,Bmi-1基因的RNA干扰显著增加了体外培养的NSC的凋亡率。结论 Bmi-1基因对于体外培养的NSC具有抑制其凋亡的作用,在分析该基因对NSC增生、自我更新等生物学属性的影响时,应当将Bmi-1基因的抗凋亡作用考虑在内。Objective To explore the effect of Bmi-1 on the apoptosis of human fetal striatum derived neural stem cell （NSC）. Methods The NSC was isolated from the striatum of 16-18 week fetus suffered from accidental spontaneous abortion, cultured and passaged 3-5 times in vitro. Bmi-1RNA interference and rescue （ over-expression of Bmi-1 ） experiments were performed by lentivirus. The effects of Bmi-1 on the apoptosis of NSC were then observed. Results The Bmi-1 transcription of human striatum derived NSCs was decreased significantly by RNAi and was reversed by rescue experiments （overexpression of Bmi-1 ）. Correspondingly, both at the time points of 2h and 3d after passaging, the RNAi of Bmi-1 increased the apoptosis significantly. Conclusion Given human fetal striatum derived NSCs, Bmi-1 inhibited their apoptosis in vitro. In the cases studying the effect of Bmi-1 on the self-renewal, proliferation of NSC, the anti-apoptosis effect of Bmi-1 should be taken into consideration.

关 键 词：神经干细胞 凋亡 BMI-1 癌基因 

分 类 号：R392.2[医药卫生—免疫学]