miR-125a-3p和miR-17-5p在激素性股骨头坏死中的作用  

Effects of miR-125a-3p and miR-17-5p on steroid-induced osteonecrosis

作　　者：吴兴净[1] 张永涛[1] 郭雄[2] 王坤正[1] 

机构地区：[1]西安交通大学医学部第二附属医院骨科,陕西西安710004 [2]西安交通大学公共卫生学院环境与基因相关疾病重点实验室,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2015年第2期210-214,共5页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金(No.81371944)~~

摘　　要：目的探讨激素性股骨头坏死中miRNA的差异性表达变化。方法取激素性股骨头坏死的坏死区域骨组织为实验组,其股骨颈基底部骨组织为对照组;分别提取总RNA并进行质检;采用miRNA芯片技术检测miRNAs的表达并对其表达谱进行分析;运用实时定量PCR对差异明显的miR-125a-3p和miR-17-5p进行验证。结果对激素性股骨头坏死中miRNA表达谱进行差异性分析。和对照组相比,实验组中倍数大于2倍,P<0.05的miRNA共有11个。8个miRNA表达上调,3个表达下调。实时定量PCR证实miR-125a-3p在坏死标本中高表达,而miR-17-5p呈现低表达,与miRNA芯片结果一致。结论激素性股骨头坏死区域骨组织与对照组相比miRNAs表达发生明显变化,以miR-125a-3p和miR-17-5p差异最为明显。这表明了它们最有可能参与了激素性股骨头坏死病理过程的调控。Objective To explore the differential expression of microRNAs （miRNAs）in steroid-induced osteonecrosis （ON）.Methods Bone tissues were selected from patients with steroid-induced ON between the necrotic zone of femoral heads and its femoral neck to analyze the miRNA expression profile using the microarray. The most differentially expressed miR-125a-3p and miR-1 7-5p in microarray analysis were further confirmed by real-time quantitative PCR.Results According to the microarray screening,8 miRNAs were upregulated and 3 miRNAs were downregulated in the necrotic zone of femoral heads samples （Fold〉2,P 〈0.05 ）.Results of real-time PCR revealed that miR-125a-3p was upregulated and miR-1 7-5p was downregulated in the necrotic zone of femoral heads samples,which were in agreement with the microarray data.Conclusion MiRNA’s differential expression profile of human steroid-induced ON samples was obtained.Among the differentially expressed miRNAs, miR-125a-3p and miR-1 7-5p are the most apparently differentially expressed miRNAs,which may be related to the pathogenesis and development of steroid-induced ON.

关 键 词：激素性股骨头坏死 miR-125a-3p miR-17-5p MIRNA MIRNA芯片 

分 类 号：R681.8[医药卫生—骨科学]