糖尿病性勃起功能障碍大鼠模型的建立  

Establishment of rat model of diabetic erectile dysfunction

作　　者：曹石金[1] 谢礼仁 朱斌[1] 金岩[1] 潘卫兵[1] 

机构地区：[1]深圳市坪山新区人民医院泌尿外科,广东深圳518118

出　　处：《解剖学研究》2015年第1期15-17,共3页Anatomy Research

基　　金：深圳市坪山新区医疗卫生发展孵化资金(201206);深圳市科技创新委员会知识创新计划基础研究(201404163000117)

摘　　要：目的探讨理想糖尿病性勃起功能障碍(ED)大鼠模型的制备方法。方法90只SD大鼠,随机分为Ⅰ、Ⅱ、Ⅲ组,每组再分为正常组(CN)和糖尿病组(DM),每组各15只,糖尿病组腹腔注射链脲佐菌素(STZ,65 mg/kg)诱导糖尿病大鼠模型,成模8、12及16周注射阿朴吗啡(APO,80μg/kg)观察大鼠阴茎勃起情况,制备糖尿病性ED大鼠模型。结果DM组在8、12、16周注射阿朴吗啡后勃起次数分别为1.0±0.0、1.0±0.0、1.0±0.0,勃起率分别为33.3%、21.4%、14.3%,ED发生率分别为66.7%、78.6%、85.7%,CN组勃起次数分别为2.2±0.8、2.3±0.8、2.0±0.7,勃起率均为100%,ED发生率为0。组间差异有统计学意义(P<0.01)。结论采用STZ 65 mg/kg腹腔注射建立糖尿病大鼠模型方法安全有效,颈部皮下注射APO 80μg/kg筛选糖尿病ED大鼠可行可靠。Objective To investigate the ideal diabetic rats model with erectile dysfunction.Methods Ninety male SD rats randomly divided into 3 groups（groupⅠ,Ⅱ,Ⅲ）.Each group equally into control group and diabetes mellitus group again,15 rats in each group.SD rats were injected intraperitoneally with STZ to make experimental models of DM.Injected with apomorphine,penile erection of the rat models with DM and the controls were observed and noted on the 8th,12 th,and 16 th week from the 3rd day of STZ injection.Results The DM ED rat model was established after 8,12,and 16 weeks,the rate with pe nis erection in diabetic group was only 33.3%,21.4%,14.3% respectively,which was much lower than that 100% in the control groups（P〈0.01）.Conclusion Intraperitoneal injection with STZ（65 mg / kg） was a safe method for establishment of diabetic rat model.Subcutaneous injection with Apomorphine（80 μg / kg） was an effective method to screen diabetic rat model with erectile dysfunction.

关 键 词：糖尿病 勃起功能障碍 链脲佐菌素 

分 类 号：R587.2[医药卫生—内分泌]