几丁质微粒对中性粒细胞为主支气管哮喘小鼠气道炎症的影响  

Effects of chitin micro-particles on airway inflammation in a mouse neutrophilic asthmatic model

作　　者：周作人[1] 潘频华[1] 谭洪毅[1] 胡永斌[2] 胡成平[1] 

机构地区：[1]中南大学湘雅医院呼吸与危重症医学科,长沙410008 [2]中南大学湘雅医院病理科,长沙410008

出　　处：《中华结核和呼吸杂志》2015年第3期185-190,共6页Chinese Journal of Tuberculosis and Respiratory Diseases

基　　金：国家自然科学基金面上项目（81170023）;十二五国家科技支撑计划课题（2013BAI09809）;中南大学研究生自主创新项目（2013zzts323）

摘　　要：目的 研究几丁质微粒（CMP）对中性粒细胞为主支气管哮喘（NA）小鼠气道炎症和细胞因子IL-10及IL-17的影响.方法 将30只4～6周龄的BALB/c小鼠按随机数字表法分为5组：健康对照组、CMP对照组（CMP干预,PBS致敏,PBS激发）、NA组[PBS干预,卵清白蛋白（OVA）＋内毒素致敏,OVA激发]、CMP治疗组（CMP干预,OVA＋内毒素致敏,OVA激发）、地塞米松（DXM）治疗组（DXM干预,OVA＋内毒素致敏,OVA激发）,每组6只.末次激发24 h后测气道阻力,收集BALF、血清,并取肺组织行HE染色,BALF瑞氏染色白细胞分类计数,ELISA法检测BALF及肺组织匀浆细胞因子IL-17及血清IL-10含量.结果 （1）肺组织病理：NA组支气管周围、血管周围大量炎症细胞浸润,CMP治疗组气道炎症明显轻于NA组及DXM治疗组,重于健康对照组及CMP对照组.（2） BALF细胞分类计数：BALF中性粒细胞CMP治疗组[（5.1 ±2.0） ×105/ml]明显低于NA组[（9.2 ±2.0）×10^5/ml]及DXM治疗组[（7.9 ±2.3） ×10^5/ml],高于健康对照组[（1.4±0.4）×10^5/ml]及CMP对照组[（1.5±0.7）×10^5/ml],差异有统计学意义（F =27.1,P＜0.05）.（3）CMP治疗组血清IL-10含量明显升高[（80±16） ng/L],高于NA组[（15±8）ng/L]、健康对照组[（29±10） ng/L]、CMP对照组[（28±7）ng/L]及DXM治疗组[（18±10） ng/L],差异有统计学意义（F=37.1,P＜0.05）.（4） CMP治疗组BALF中IL-17[（86±10） ng/L]明显低于NA组[（111±11）ng/L]及DXM治疗组[（101 ±9） ng/L],高于健康对照组[（63±10）ng/L]、CMP对照组[（65±8）ng/L],差异有统计学意义（F=32.2,P＜0.05）.结论 CMP可能降低气道IL-17而减轻NA小鼠的中性粒细胞气道炎症.Objective To investigate the impact of chitin micro-particles （CMPs） on the airway inflammation and the cytokine levels of IL-10 and IL-17 in mice with neutrophilic asthma （NA）.Methods Thirty 4-to 6-week-old BALB/c mice were divided into 5 groups based on random number table：healthy control group,CMP control group （CMP intervention,PBS sensitization and PBS challenge）,NA group （PBS intervention,OVA ＋ LPS sensitization and OVA challenge）,CMP treatment group （CMP intervention,OVA ＋ LPS sensitization and OVA challenge）,and DXM treatment group （DXM intervention,OVA ＋ LPS sensitization and OVA challenge）.Each group included 6 mice.Twenty four hours （24 h） after the last challenge,airway resistance was measured,bronchoalveolar lavage fluid （BALF） and serum were collected,and lung tissue was taken for HE staining.Wright＆#39; s staining was performed on BALF for white blood cell differential count.The ELISA method was used to determine the content of cytokine IL-17 in BALF and lung tissue homogenate and the content of cytokine IL-10 in serum.Results A large number of inflammatory cells infiltrated the surroundings of bronchus and blood vessel in the NA group.The airway inflammation in the CMP treatment group was significantly milder than that in the NA group and the DXM treatment group,but more severe than that in the healthy control group and the CMP control group.The number of BALF neutrophils in the CMP treatment group [（5.1 ±2.0） × 105/ml] was significantly lower than that in the NA group [（9.2 ±2.0） × 10^5/ml] and the DXM treatment group [（7.9 ± 2.3） × 10^5/ml],but higher than that in the healthy control group [（1.4 ± 0.4） × 10^5/ml] and the CMP control group [（1.5 ± 0.7） × 10^5/ml],the difference being statistically significant （F =27.1,P 〈0.05）.The content of IL-10 in serum in the CMP treatment group increased significantly [（80 ± 16） ng/L],and was higher than that in the NA group [（15 ± 8） ng/L],the healthy contrd group [（29

关 键 词：哮喘 炎症 几丁质 中性粒细胞 

分 类 号：R562.25[医药卫生—呼吸系统]