大鼠脑挫伤后脑C-FOS、AQP-4表达与损伤时间的关系  

Study on the relationship between contusion time and expressions of C-FOS & AQP-4 in rat brain after cerebral contusion

作　　者：胡玉莲[1,2] 许光亚[1,3] 刘敏[1] 

机构地区：[1]四川大学华西基础医学与法医学院,四川成都610041 [2]第三军医大学西南医院医教部,重庆400038 [3]四川华大司法鉴定所,四川成都610041

出　　处：《中国法医学杂志》2015年第1期24-28,共5页Chinese Journal of Forensic Medicine

摘　　要：目的 观察大鼠脑挫伤组织周围C-FOS、AQP-4表达的时序性变化规律,分析评价其在脑挫伤时间推断中的价值.方法 采用自由落体撞击法建立SD大鼠开放性脑挫伤模型,于伤后0～168h不同时间点取挫伤区脑组织,应用HE染色及免疫组织化学染色方法,结合图像分析及统计学技术进行分析,并与假手术组和正常对照组进行比较.结果 大鼠脑挫伤后0～ 168h时间内脑皮质挫伤区内C-FOS、AQP-4的表达随损伤时间的变化呈明显的时序性变化,C-FOS阳性表达于伤后12h、AQP-4阳性表达于伤后72h分别达高峰.根据实验组各时间点IOD值,分别建立根据C-FOS、AQP-4表达推断损伤时间的回归方程.结论 脑挫伤部位C-FOS和AQP-4的表达规律在脑挫伤时间推断中有潜在的应用价值.Objective To observe the sequential change rule of expressions of C-FOS and AQP-4 in rat brain after cerebral contusion, and we use statistical regression analysis to explore the advantages of the combination of the two factors in contusion time estimation. Methods We use Sparague-Dawely rats to set the cerebral contusion model by using free-fall crash, and HE staining and immunohistochemical methods were used to explore the expression of C-FOS and AQP4, then analyze the expression data with image analytical system and homologous statistical statistics. We found the expression regulation with comparison and analysis the operated group, sham-operated group and normal control. Results Expressions of C-FOS and AQP4 in cerebral cortex contusion showed significant sequential changes by the contusion time within 168 hours. C-FOS positive expression rocketed to the peak in 12h, while positive expression of AQP-4 increased slowly, reached to the peak at 72 hours. Based on this data of IOD, we established regressive equation of expressions of C-FOS and AQP-4 in cerebral contusion time. Conclusion The expressions regulation of C-FOS and AQP4 in rat brain after cerebral contusion have potential applications in estimating the contusion time.

关 键 词：法医病理学 脑挫伤 C-FOS AQP-4 损伤时间推断 免疫组织化学 

分 类 号：D919[医药卫生—法医学]